中药钩藤不同药用部位异钩藤碱含量分析 - 国际中医中药杂志.PDF

·258 ·                                   国际中医中药杂志2015年3月第37卷第3期 Int J Trad Chin Med, March 2015, Vol. 37, No.3  ·论著 · 中药钩藤不同药用部位异钩藤碱 含量分析 王盟 赵亚鑫 【摘要】 目的 测定钩藤的不同药用部位中异钩藤碱的含量。方法 应用高效液相色谱法(HPLC) 测定 8 批不同产地钩藤的不同药用部位中异钩藤碱含量。采用 Waters Symmetry C18 色谱柱（250 mm × 4.6 mm ，5 μm ），流动相为甲醇-0.01 mol/L 醋酸铵缓冲液（pH 8.0 ）（60 ∶40 ），柱温25 ℃，进样量20 μl， 流速 1.0 ml/min，检测波长246 nm 。结果 8 批不同产地钩藤药材中均能检测出异钩藤碱，但产地间差异 较大；不同药用部位中异钩藤碱的含量：主杆＞带钩茎枝＞无钩茎枝＞无茎枝钩＞叶。结论 钩藤不同 药用部位大部分含异钩藤碱成分，为扩展钩藤植物的药用部位提供试验依据。 【关键词】 钩藤；药用部位；含量分析；异钩藤碱 Analysis of the contents of isorhynchophylline in different medicinal parts of Ramulus Uncariae Cum Uncis Wang Meng, Zhao Yaxin. NO.1 People’s Hospital of Shandong Jining, Jining 272111, China Corresponding author: Zhao Yaxin, Email:163.com 【Abstract 】 Objective To explore the content of isorhynchophylline in different medicinal parts of Ramulus Uncariae Cum Uncis. Methods HPLC was adopted to determine 8 batches of different Ramulus Uncariae Cum Uncis of different origin and different medicinal parts. The Waters Symmetry C18 color （4.6 mm×250 mm, 5 μm ） was used with mobile phase of methanol -0.01 mol/L ammonium acetate buffer （pH 8.0 ）（60 ∶40 ）, column temperature of 25 ℃ , 20 μl sample volume, velocity of 1.0 ml/min, and detection wavelength of 246 nm. Results Isorhynchophylline can be detected in all 8 batchs of Ramulus Uncariae Cum Uncis in different regions, and different content were found among different origins. The content of isorhynchophylline in different parts of the same origin showed a decreasing sequence of the rhabd, stem with hook, stem without hook, and twig without hook and leaves. Conclusion Most of medicinal part of Ramulus Uncariae Cum U