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微波辅助提取银杏叶多糖工艺及其体外抗氧化活性研究
24 2012, Vol. 33, No. 06 食品科学 ※工艺技术
陈义勇,冯燕红
(常熟理工学院生物与食品工程学院,江苏 常熟 215500)
以水作为提取溶剂、银杏叶多糖提取率为指标,采用微波辅助提取法,在单因素试验的基础上,通过
正交试验对银杏叶多糖的微波辅助提取工艺进行优化,并采用清除 DPPH 自由基、·OH 和 O2 ·模型对其体外抗
氧化活性进行评价,并与 VC 进行比较。结果表明:微波辅助提取银杏叶多糖的最佳出工艺条件为微波功率 480W 、
液料比 30:1(mL/g) 、提取时间 8min 、提取2 次,多糖得率为 14.70%。银杏叶多糖具有较强的清除 D P P H 自由
基、·OH 的能力,并与质量浓度呈一定正相关关系,清除O2 ·能力弱,清除率与多糖质量浓度的关系不显著。
微波辅助提取;银杏叶多糖;体外抗氧化活性
Optimization of Microwave-assisted Extraction Process for Polysaccharides from Ginggo biloba Leaves and
Anti-oxidant Activity in Vitro
CHEN Yi-yong ,FENG Yan-hong
(School of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, China)
Abstract:Orthogonal array design method was applied to optimize the microwave-assisted water extraction of polysaccharides
from Ginggo biloba leaves (GBLP) in order to maximize polysaccharide yield. Meanwhile, the antioxidant activity in vitro of
polysaccharides from Ginggo biloba leaves was assessed by DPPH, hydroxyl and superoxide anion free radical scavenging assays
and compared with vitamin C. The results showed that the optimal extraction conditions were microwave power of 480 W,
material-to-liquid ratio of 1:30 (g/mL), and microwave treatment time of 8 min. Under these conditions, the extraction yield of
polysaccharides was up to 14.70%. The extract obtained had a powerful scavenging activity against DPPH and hydroxyl free
radicals in a concentration-dependent manner but a weak superoxide anion free radical scavenging activity with no significant
concentration-dependent relationship.
Key words :microwave-assisted extrac
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