标记免疫分析在农兽药残留检测中的应用研究进展 - 中国农学通报.pdf

中国农学通报 2012,28(30):218-225 Chinese Agricultural Science Bulletin 标记免疫分析在农兽药残留检测中的应用研究进展 杨代凤，刘腾飞，邓金花，顾俊荣，董明辉 （苏州市农业科学院，江苏苏州215155 ） 摘 要：为了解标记免疫分析在农药和兽药残留检测中的应用发展情况。介绍了放射免疫分析、酶免疫 分析、荧光免疫分析、化学发光免疫分析以及胶体金免疫分析等标记免疫技术的基本原理，总结了这5 种标记免疫技术的优缺点，概述了其在农药和兽药残留检测中的应用，并探讨了标记免疫分析存在的问 题及未来的发展趋势。结果表明：标记免疫分析操作简便、特异灵敏、快速准确，能够为保证农产品的食 用安全性提供有力的技术支撑，今后将成为农兽药等药物残留检测的主要技术之一。 关键词：标记免疫分析；农药和兽药；残留；快速检测 中图分类号：R446.6 ，S481 ，S859 文献标志码：A 论文编号：2012-2424 Development and Application of Labeled Immunoassay in Pesticide and Veterinary Drug Residues Analysis YangDaifeng,LiuTengfei,DengJinhua,GuJunrong,DongMinghui (SuzhouCityAcademyofAgriculturalSciences,SuzhouJiangsu215155) Abstract: Tounderstandthedevelopmentandapplicationoflabeledimmunoassaysinthedeterminationof pesticideandveterinarydrugresidues,thebasicprinciples,advantagesanddisadvantagesoffivecommon labeled immunoassays including radioimmunoassay, enzyme immunoassay, luminescent immunoassay, fluorescenceimmunoassayandcolloidalgoldimmunoassaywereintroducedandsummarizedinthispaper,and theirapplicationsindeterminationofpesticidesandveterinarydrugsinrecentyearsweredescribedaswell. Additionally,someexistingproblemsandfuturetrendsoftheseimmunoassaysforpesticidesandveterinary drugsanalysiswerediscussed.Theresultsshowedthatthedescribedaboveimmunoassayswereprovedtobe simple,rapid,accurate,andofhighspecificityandsensitivity.Itwasbelievedthatinthenearfuturetheycould probablybecomeoneofthemostpromisingmethodsfordetectingresidualpesticidesandveterinarydrugsand providepowerfultechnicalsupportfortheediblesecurityofagriculturalproducts. Key words: labeledimmunoassay;pesticideandveterinarydrug;residues;rapiddetection 0 引言 造