dys19 、dys391 、dys439 三个y-str 荧光标记复合扩增的法医学 .pdfVIP

DOI :10.16467/j.1008 -3650.2004.04.004 2004 4 ·· DYS19、DYS391、DYS439 Y-STR 1, 2 2 3 4 5 (1. , 430019;2. 朱传红 , 杨庆恩, 王海生, 申成斌, 刘成昌 　 , 　430030;3. , 　430070;4. , 　460010;5. , 466000) :　研 Y—染色体 STR 基因座在法医学检测中的应用价值。　用荧光标记 DYS19,DYS391, DYS439 三 个Y—STR基因座,PCR 复合扩增,通过毛细管电泳得到结果。　三 个Y—STR 基因座有较高 的种属特异性;观察50次男性配子细胞形成过程中的减数分裂未发现突变基因;对男∶女不同比例混合血样检 测,当男∶女性血样比达1 ∶50 时,仍能准确分型Y—STR 基因型,并且Y—STR 检验较常染色体STR 分型更有 优势;检测了1～15 个月病理石蜡切片,表明Y-STR基因座适合降解DNA 的检测。　Y-STR 分型适合 日常法医检案的需要,该方法是对常染色体STR 应用的一个补充。 :Y 染色体;STR;复合扩增;毛细管电泳;法医学应用 :DF795.2　　:A 　　:1008-3650(2004)04-0011-04 The investigation on forensic application of DYS19, DYS391, DYS439 by multiplex PCR an capillary electro- phoresis Department of Medicolegal, Tongj i Medical College, ZHU-Chuan-hong, YAN Qing-en, WAN Hai-sheng, et al.( Huazhong Science and Technology University, Wuhan 43 3 , China) ABSTRACT:　Objective　In order to evaluate the value of three Y chromosome STRs in forensic science.Metho s　A multiplex PCR to amplify three Y-chomosome STRs loci in the same PCR reaction was set up.PCR products were analyzed using capillary elec- trophoresis and eneScan Software on the ABI Prism 310 DNA sequencer.A study of forensic application of three Y-ST R loci were also carried out to evaluate the useful value in cases investigation which include determination of the sensitivity, cross reactivity from species samples other than human and genotyping DNA different tissues of the same individual.Furthermore, DNA genotyping of three Y-STR of bloodstain were carried out which different proportion of male and female blood were mixed.Results　Family investigation confirmed that DYS19, DYS391, DYS439 were in accordancewith Mendelian inheritance.No mutations were detected in 50 meiosis of germline analysis.PCR with mixtures of male/female DNA showed that male