雷公藤红素经过TRAIL 途径增强酌啄T 细胞对骨肉瘤细胞株HOS 的.PDF

李朝旭等摇 雷公藤红素经过TRAIL途径增强酌啄T细胞对骨肉瘤细胞株HOS 的杀伤作用摇 第12期 ·1777 · doi:10.3969/ j.issn.1000鄄484X.2016.12.011 ·中医中药与免疫 · 雷公藤红素经过TRAIL途径增强酌啄T细胞对骨肉瘤细胞株HOS 的 杀伤作用淤 于 李朝旭摇 张浚哲摇 王胜涛摇 王锐英摇 唐际存 摇 (桂林医学院附属医院骨二科,桂林 541004) 摇 摇 中图分类号摇 R392郾12摇 摇 文献标志码摇 A摇 摇 文章编号摇 1000鄄484X(2016)12鄄1777鄄04 [摘摇 要]摇 目的:探讨雷公藤红素经过TRAIL途径增强酌啄T细胞对骨肉瘤细胞株HOS的杀伤作用。 方法:Western blot 检测骨肉瘤细胞株HOS死亡受体(DR)的表达。 使用唑来膦酸(ZOL)联合IL鄄2体外扩增人酌啄T细胞,LDH法检测酌啄T细胞 对HOS细胞株的杀伤活性。 结果:雷公藤红素可促进骨肉瘤细胞株HOS死亡受体DR4 和DR5 的表达(P0郾05)。 健康人来 源的酌啄T细胞具有杀伤HOS细胞株的能力(P0郾05),经过雷公藤红素(1 滋mol/ L)24 h预处理HOS细胞株,酌啄 T细胞杀伤 HOS细胞株的能力增强(P0郾05),TRAIL 中和抗体可阻断雷公藤红素增强酌啄T细胞杀伤HOS细胞株的能力(P0郾01)。 结 论:雷公藤红素经过TRAIL途径增强酌啄T细胞对骨肉瘤细胞株HOS的杀伤作用。 [关键词]摇 骨肉瘤;酌啄T细胞;免疫治疗;雷公藤红素 Celastrol increases osteosarcoma cells line HOS lysis by 酌啄 T cells through TRAIL way LI Zhao鄄Xu,ZHANGJun鄄Zhe,WANGSheng鄄Tao,WANGRui鄄Ying,TANGJi鄄Cun郾Departmentof OrthopaedicsNo郾2, Affiliated Hospital of GuilinMedical University,Guilin541004,China [Abstract]摇 Objective:To investigate whether celastrol can increase the cytotoxic activity of 酌啄 T cells against osteosarcoma (OS) cells line HOS throughTRAILway郾Methods:Thedeathreceptors4/ 5 (DR4/ 5)proteinlevelsintheOScelllinesHOSwasin鄄 vestigated by Western blot analysis郾Zoledronate (ZOL)wasusedtoinduce酌啄T cellsfromPBMCsof healthyvolunteers郾酌啄T cell cy鄄 totoxicity against HOS cells was investigated by a standard lactate dehydrogenase release assay (LDH)郾Results:Celastrol increased DR4/ 5 protein expression in HOS (P0郾05)郾酌啄 T cells from PBMCs of healthy volunteers showed cytotoxicity against HOS (P 0郾05).Following co鄄culture with HOS pre鄄treated with celastrol (1 滋mol/ L) for 24 h,酌啄 T cells showed significantly higher cytotoxicity against HOS (P0郾05)郾The induction of DR4/ 5 molecules increased lysis of HOS by 酌啄 T cells which was abolished by addition of a blocking TRAIL antibody郾Conclusion:Celastrol can enhance 酌啄 T cells忆cytotoxic activity against