Antibody-antigen binding kinetics a model for multivalency antibodies for large antigen systems：（Antibody-antigen绑定动力学模型多价大型抗原抗体系统）.pdfVIP

Copyright 9 1997by Humana Press Inc. All rights of any nature whatsoever reserved. 0273-2289/97/6701-02~0001513.25 Antibody-Antigen Binding Kinetics A Model for Multivalency Antibodies for Large Antigen Systems AJIT SADANA12AND TUAN Vo-DINH *1 1AdvancedMonitoring DevelopmentGroup, Life Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 2Chemical EngineeringDepartment, Universityof Mississippi, University,MS ReceivedAugust 7, 1996;Accepted November 11, 1996 ABSTRACT This work presents a theoretical analysis of the influence of multi- valency of antigen on external mass transfer-limited binding kinetics to divalent antibody for biosensor applications to polycyclic-aromatic sys- tems. Both cases are considered wherein the antigen is in solution and the antibody is either covalently or noncovalently attached to a cylindri- cal fiber-optic biosensor, and the antibody is in solution and the antigen is attached to the surface. Both single-step and dual-step binding processes are considered. The rate of attachment of antigen to antibody (or vice versa) is linear for the valencies (or reaction orders) analyzed in the time frame (100 min) considered. The rate of attainment of saturation levels of antigen or antibody in solution close to the surface is very rapid (within 20 min). An increase in the valency of the antigen in solution has the effect of decreasing the order of reaction (for valency, v 1). An increase in the number of steps increases the order of reaction, as expected. An increase in the valency of the antigen in solution decreases the saturation level of the antigen close to the surface and the rate of anti- gen attachment t