高通量筛选的酶活性测定.pdfVIP

Enzyme assays for high-throughput screening Jean-Philippe Goddard and Jean-Louis Reymond Assaying enzyme-catalyzed transformations in high- New enzyme assays throughput is crucial to enzyme discovery, enzyme engineering Umbelliferone and nitrophenol and the drug discovery process. In enzyme assays, catalytic Umbelliferone is a prototypical fluorogenic phenol. Esters activity is detected using labelled substrates or indirect sensor and ethers of umbelliferone are non-fluorescent, whereas systems that produce a detectable spectroscopic signa