冷榨茶叶籽油甘三酯组成分析.pdfVIP

现代食品科技 Modern Food Science and Technology 2013, Vol.29, No.4 冷榨茶叶籽油甘三酯的组成分析 1 1 2 韦利革 ，李桂华 ，谢明 （1.河南工业大学粮油食品学院，河南郑州 450001）（2.浙江泰谷农业科技有限公司，浙江杭州 310000） 摘要：本文采用气相色谱法测定了茶叶籽油的脂肪酸组成，得出茶叶籽油中不饱和脂肪酸含量在80%以上，主要是以油酸的形 式存在，含量为59.84% ；采用胰脂酶水解法测定茶叶籽油的Sn-2位脂肪酸分布，并根据1,3-随机-2-随机分布学说计算出茶叶籽油甘三 酯组成, 得出主要甘三酯是OOO 、OOL、POO、PLO 、LLO等，其中三不饱和脂肪酸甘油酯含量在53.5%以上，而三饱和脂肪酸甘油 酯含量仅为0.05%左右；由高效液相色谱测定得出茶叶籽油中的维生素E含量为0.18 mg/g；Rancimat仪测定茶叶籽油氧化稳定时间在 110 ℃时为6.02 h 。茶叶籽油具有很好的食用营养价值和储藏稳定性。 关键字：茶叶籽油；脂肪酸组成；甘三酯结构；维生素E ；氧化稳定时间 文章篇号：1673-9078(2013)4-911-915 Analysis of Triglycerides of Cold Precessed Tea Seed Oil 1 1 2 WEI Li-ge , LI Gui-hua , XIE Ming (1.Department of Agriculture and Food, Henan University of Technology, Zhengzhou 450001, China) (2.Zhejiang TAIGOL Agricultural Development Co ., Ltd, Zhejiang 310000, China) Abstract: The fatty acid composition of tea seed oil was detected by using Gas Chromatography. It was found that tea seed oil contained unsaturated fatty acid up to 80%, the major of which were oleic acid and linoleic acid. By the method of pancreas lipase hydrolysis , the Sn-2 position fatty acid was analyzed and the triglycerides composition of tea seed oil were calculated according to 1,3-random-2-random distribution doctrine. The key triglycerides were found as OOO, OOL, POO, PLO, LLO and PPO. Among them, the content of three saturated fatty acid esters was 53.5%, and the content of three saturated fatty acid ester content was only 0.05%. Through HPLC and Rancimat determination, vitamin E content of the tea seed oil was found as 0.18 mg/g and the duration of the oxidation stability at 110 ℃w