显齿蛇葡萄叶提取物对大鼠肝癌前病变模型抗氧化酶活性的影响钟正贤.doc.docVIP

DOI:10.13862/j.cnki.cn43-1446/r.2009.08.027 第15卷 第8期 2009 年 8 月 Vol.15 No. 8 Guiding Journal of Traditional Chinese Medicine and Pharmacy Augusty.2009 !!!!! !科研报告 ! !!!!! 显齿蛇葡萄叶提取物对大鼠肝癌前病变模型抗氧化酶活性的影响* 钟正贤1，陈学芬1，李燕婧1，杨秀芬2 （1.广西中医药研究院，广西 南宁 530022；2.广西中医学院药学院，广西 南宁 530002) [摘要] 目的：探讨显齿蛇葡萄叶提取物在大鼠肝脏化学致癌初期对血清及肝脏抗氧化酶活性的影响，从而进一步探索其抗致癌作用的可能机制。方法：按改良的Solt Farber方法建立大鼠肝癌前病变模型，用显齿蛇葡萄叶水提物及总黄酮饲养大鼠6周后，取血及肝脏测定超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-px)、谷胱甘肽-S-转移酶(GSH-ST)、过氧化氢酶(CAT)、丙二醛(MDA)及r-谷氨酰转肽酶(r-GT)。结果：显齿蛇葡萄叶水提物及总黄酮在大鼠肝脏化学致癌初期对血清和肝脏中SOD、GSH-px、GST、CAT的活性有明显回升作用，降低MDA含量，并能抑制癌前病变标志物r-GT活性表达。结论：显齿蛇葡萄叶提取物具有抗氧化和抑制癌前病变标志物的作用，可能是显齿蛇葡萄叶的抗致癌机制之一。 [关键词] 显齿蛇葡萄叶；化学致癌；抗氧化酶；大鼠 [中图分类号] R285.5 [文献标识码] A [文章编号] 1672-951X（2009）08-0001-03 Effects of Ampelopsis Grmsedntara Fxtract on the Serum and Liver Antioxidative Activities in Initial Stage of Rat Chemical Hepato-carcinogenesis ZHONG Sheng-xian1，CHEN Sue-fen1，LI Yan-jing1，YANG Xiu-fen2 1.Guangxi Institute of Traditional Chinese Medicine，Nanning,China,530022; 2.Guangxi Tradition Chinise Medical College Nanning,China, 530002 [Abstract] Objective：To study effect from extract of Ampelopsis grmsedntara （AGE）on serum and liver antioxidative ac－ tivities and r-glutamine trans- peptidase (r-GT) activity in initial stage of rat chemical hepato- carcinogenesis and invesdgate possible mechanism of its anti- carcinogenesis． Methods：Hepatic preneoplastic lesions in rats were induced by modified SOh-far－ ber protoco1． The activities of serum superoxide dismutase(SOD)，glutathione peroxidase(GSH-px)，glutathione-S-transferase(GST)， catalase (CAT), r -glutamine transpeptidase (r -GT) and the content of malondialdehyde(MDA)were measured with colorimetric method in the rats administered with water extract and total flavone for 6 weeks． Results：The activities of serum and liver SOD,GSH-px, GST,CAT were increased, decreasing the content of MDA and r-GT activity of initial stage of rat chemical hep － ato- carcinogenesis marke