糖原合成酶激酶3β过表达心肌干细胞移植治疗心肌梗死.doc.docVIP

糖原合成酶激酶3β过表达心肌干细胞移植治疗心肌梗死 引用本文：赵翠花，李彦明，钟晓鸣，何瑞利，程冠昌. 糖原合成酶激酶3β过表达心肌干细胞移植治疗心肌梗死DOI: 10.3969/j.issn.2095-4344.2016.41.019 ORCID: 0000-0001-7665-9758(程冠昌) 文章快速阅读： 文题释义： 糖原合成酶激酶3β(GSK-3β)β在心3β(GSK-3β)与细胞的各项生命活动之间存在密切的联系，关于其在心肌干细胞向心肌细胞分化中的作用机制和效果尚不清楚。 目的：探讨GSK-3β过表达心肌干细胞移植治疗心肌梗死大鼠的效果。 方法：分离培养乳鼠心肌干细胞，干细胞使其过表达GSK-3β或对照基因LacZ。30只SD大鼠制备心肌梗死模型随机分为3组：GSK-3β组、LacZ组、PBS组。模型制备后6周，于梗死心肌内局部注射过表达GSK-3β、LacZ心肌干细胞以及等量PBS。移植后4周，检测心功能及心室胶原生成情况。 -3β组的左室射血分数显著大于其他两组(P 0.05)，左室舒张末内径显著小于其他两组(P 0.05)；②GSK-3β组羟脯氨酸含量、Ⅰ型胶原mRNA、Ⅲ型胶原mRNA表达显著小于其他两组(P 0.05)；③经Masson染色发现，GSK-3β组的心室肌蓝染的胶原含量明显低于LacZ组；④GSK-3β组的心肌梗死范围显著小β能激酶3β：glycogen synthase kinase-3β，GSK-3βOverexpression of glycogen synthase kinase 3beta in the treatment of myocardial infarction with cardiac stem cell transplantation Zhao Cui-hua, Li Yan-ming, Zhong Xiao-ming, He Rui-li, Cheng Guan-chang (Department of Cardiovascular Medicine, Huaihe Hospital of Henan University, Kaifeng 475000, Henan Province, China) Abstract BACKGROUND: The mechanism and effect of glycogen synthase kinase 3β (GSK-3β) in the differentiation of cardiac stem cells into cardiomyocytes are still unclear, although GSK-3β is closely related to the life activities of cells. OBJECTIVE: To investigate the changes of GSK-3β expression in the treatment of myocardial infarction in rats undergoing cardiac stem cell transplantation. METHODS: The isolation and culture of cardiac stem cells were performed in 10 neonatal rats. Lentivirus overexpressing GSK-3β or LacZ (control) was constructed and transferred into cardiac stem cells. Animal model of myocardial infarction was made in 30 Sprague-Dawley rats. Six weeks after model preparation, rat models were assigned into GSK-3β, LacZ or PBS group. GSK-3β or LacZ overexpressing cardiac stem cell solution or PBS in equal volume was injected into the rat myocardium, respectively. Four weeks after transplantation, the cardiac function and myocardial collagen production in rats were detected and compared. RESULTS AND CONCLUSIO