26 423-425 食品安全与检测.转420.2009112固相萃取毛细管电泳法测 定奶粉中三聚氰胺的含量4119A-X20090141.pdfVIP

现代食品科技 Modern Food Science and Technology 2010, Vol.26, No.4 固相萃取-毛细管电泳法测定奶粉中 三聚氰胺的含量 1 2 卢日刚 ，梁佳 （1.广西食品药品检验所，广西南宁 530001 ）（2.广西中医学院，广西南宁 530021 ） 摘要：建立了高效毛细管电泳法测定奶粉中三聚氰胺含量的方法。在背景电解质溶液为30 mmol/L 的硼砂缓冲液中加入15 mmol/L 的十二烷基硫酸钠(SDS), 运行电压为15 kV, 压力进样为10 s, 柱温为25 ℃, 检测波长为200 nm 。结果表明：三聚氰胺的线性范围20~50 μg/mL （r=0.9994 ）, 平均回收率为99.3% ，检出限为1 μg/mL，定量限为2 μg/mL。结论是本法简便快捷，结果准确可靠，可作为该成分的质量控制方法。 关键词：高效毛细管电泳法；三聚氰胺；含量 文章篇号：1673-9078(2010)4-423-425 Determination of Melamine in Milk Powder by SPE-HPCE 1 2 LU Ri-gang , LIANG Jia (1.Guangxi Institute for Food and Drug Control, Nanning 530021,China) (2.Guangxi University of Traditional Chinese Medicine, Nanning,530001,China) Abstract: A capillary electrophoresis method for the determination of melamine in milk powder was developed in this paper. The sample was analyzed with 30 mmol/L Na B O running buffer containing 15mmol/L SDS at 25 ℃. The voltage, sample injection time 2 4 7 and detection wavelength were 15 kV, 5s and 200nm, respectively. The linear response ranges, the mean recoveries and the detection limit were 20-50 μg/mL (r=0.9994) , 99.3% and 1μg/ml, respectively. The quality limit was 2μg•ml-1 for melamine. This method was simple, fast, accurate and reliable, which was suitable for quality control of the melamine. Key words: HPCE; melamine; content 423 三聚氰胺简称三胺，别名蜜胺、氰脲酰 min ，再振荡提取10 min后，以6000 胺，是一种用途广泛的具有氮杂环结构的有 r/min离心