四倍体菘蓝转抗虫基因研究.PDFVIP

·846· 　Chinese Traditional and Herbal Drugs　 34 9 2003 9 ·· Ⅱ . 1 1 1 2 2 1 1 1 许铁峰 ,张　磊 ,刘成洪 ,谭秋敏 ,唐克轩 ,郭美丽 ,乔传卓 ,张汉明 ( 1. , 　200433; 2. ,　200433) 　: 　。 　 ( PCR) , Southern RT-PCR。 　PCR Southern, , - 。 　 RT PCR DNA m RN A 。 : ;;; ; - South ern Blot RT PCR : R282. 1　　　: A　　　: 02 3 2670(2003) 09 0846 04 Ag robacterium tumef aciens mediated transgenic tetraploidIsatis ind igotica - (Ⅱ ) carrying anti insect gene fromP inellia ternata agglutinin 1 1 1 2 2 XU Tie-feng , ZHAN G Lei , LIU Cheng-hong , TAN Qiu-min , T ANG Ke-xuan , 1 1 1 GUO Mei-li , QIAO Chuan-zhuo , ZHANG Han-ming ( 1. School of Pharmacy , Second Military Medical University, Shanghai 200433, China; 2. State Key Laboratory of Genetic Enginerring, School of Life Sciences, Fudan University , Shanghai 200433, China) Abstract: Object　To get molecular proof of transgenic Isatis indigotica Fort. Methods　The regen- erated I. indigotica plantlets on kanamycin-containing media w ere analysed with polymerase chain reaction ( ) , - . 　 PCR Southern Blot and RT PCR methods Results PCR and Southern Blot analysis show ed that the . . - foreign PTA gene w ere inserted into the genome of the transformed I indigotica plants Further RT PCR show ed that the foreign PTA gene co