维生素C和E联合应用对酒精损伤睾丸超微结构的影响.docxVIP

维生素C和E联合应用对酒精损伤睾丸超微结构的影响来源：创新医学网　　作者：李宏宇,邓晶,朱玉萍,张建霞,田洪艳　　作者单位：吉林医药学院：1.临床医学院，2.护理学院，3.组织学与胚胎学教研室，吉林吉林　　【摘要】观察维生素C(VC)和维生素E(VE)联合应用对酒精(ALC)损伤睾丸超微结构的影响。方法雄性成年昆明种小鼠50只，随机分为5组：正常组、酒精组、酒精+VC组、酒精+VE组和酒精+VC+VE组，连续灌胃23 d。应用光镜和透射电镜观察睾丸组织的结构变化。结果与正常组相比，酒精组多数生精细胞核固缩，核膜模糊不清，支持细胞数量较少，细胞器结构异常;与酒精组相比，酒精+VC组多数生精细胞形态趋于正常，胞质内可见较多细胞器，支持细胞数量较多，细胞器丰富;酒精+VE组多数生精细胞固缩，细胞间隙大小不等，支持细胞数量较多，结构良好，趋于正常;酒精+VC+VE组多数生精细胞结构良好，核膜清晰，细胞器较多，支持细胞较大，核清晰，线粒体和溶酶体较多。结论 VC和VE联合应用可拮抗酒精的毒害作用，对生精小管结构具有保护作用。　　【关键词】酒精;睾丸;维生素C;维生素E;超微结构　　The effects of the joint application of vitamin C and vitamin E on the ultra structure of testis in alcoholinjured mice　　LI Hongyu1,DENG Jing1,ZHU Yuping2,ZHANG Jianxia1,TIAN Hongyan3,LI Zhixin3* (Jilin Medical College:1.Clinical Medicine College,2.Nursing College,3.Department of Histology and Embryology,JilinCity,Jilin Province,132013,China)　　Abstract:Objective To observe the effects of a joint application of vitamin C and vitamin E on the ultra structure of testis in alcoholinjured mice. Methods Fifty mature mice were divided into five groups at random:the control group;the alcohol group;the alcohol and vitamin C group;the alcohol and vitamin E group;thealcohol,vitamin C and vitamin E group.The control group was given distilled water.The other four groups were exposed to alcohol.At the same time,the alcohol and vitamin C group was given vitamin C,the alcohol and vitamin E group was given vitamin E,the last group was given both vitamin C and vitamin E.Distilledwater,alcohol,vitamin C and vitamin E were given by intragastric administration for 23 days.Light microscope and transmission electron microscope were used to detect the structure of the testis. Results Compared with the control group,nuclearpycnosis appeared in most spermatogenic cells of the alcohol group.The number of Sertoli cells decreased.The structure of organell was abnormal.Compared with the alcohol group,mostspermatogenic cells were normal in the alcohol and vitamin C group.A lot of organelles could be found