温度对中华稻蝗小分子热休克蛋白基因表达的影响 - 应用昆虫学报.pdfVIP

Chinese Journal of Applied Entomology 2016, 53(6): 12671273. DOI: 10.7679/j.issn.20951353.2016.158 温度对中华稻蝗小分子热休克蛋白基因 表达的影响* 1, 2, 4** 1, 4** 3 1, 4 1, 4 1, 4*** 史学凯 寇利花 张育平 马恩波 张建珍 吴海花 1. 030006 2. 030006 3. 030619 4. 030006 摘 要 【目的】 sHSPs sHSPs 【方法】PCR RT-qPCR Oxya chinensis 6 OcsHSPs 【结果】OcHSP19.1OcHSP19.8OcHSP20.4 OcHSP20.7 2732 37 42 42 2 h OcHSP21.1 OcHSP23.8 【结论】 sHSPs 关键词 PCR Effects of temperature on the expression patterns of OcsHSP genes in Oxya chinensis SHI Xue-Kai1, 2, 4** KOU Li-Hua 1, 4** ZHANG Yu-Ping3 MA En-Bo 1, 4 ZHANG Jian-Zhen1, 4 WU Hai-Hua 1, 4*** (1. Institute of Applied Biology, Shanxi University, Taiyuan 030006, China; 2. College of Life Science, Shanxi University, Taiyuan 030006, China; 3. Biology Department, Taiyuan Normal University, Taiyuan 030619, China; 4. Shanxi Key Laboratory of Integrated Pest Management in Agriculture, Taiyuan 030006, China) Abstract [Objectives] Small heat shock proteins (sHSPs) can be induced by environmental stress. Different temperatures can induce the expression of sHSPs genes in Oxya chinensis. [Methods] The effect of temperature on the mRNA expression patterns of six OcsHSP genes in fifth-instar nymphs of O. chinensis was analyzed using Real-time quantitative PCR. [Results] There was no significant difference in the expression levels of OcHSP19.1, OcHSP19.8, OcHSP20.4 and OcHSP20.7 at 27, 32 and 37 ℃, but expression levels significantly increased at 42 ℃, especially after 2 h at 42 ℃. There was no signif