肉穗草组织培养与扩繁研究.pdfVIP

Chinese Agricul~ral Science Bulletin Vo1．24 No．6 2008 June http：／／www．cash．org．cn ·48· 肉穗草组织培养与扩繁研究 张绪璋，周以飞，潘大仁 (福建农林大学作物学院，福州350002) 摘 要：肉穗草是一种清凉解毒，用于防治肝炎的青草药，在福建闽南一带销量较大，目前野生资源日趋 枯竭 肉穗草自然繁殖系数低，人工栽培采用分株或扦插繁殖成活率不高。采用植物组织培养技术，可以 大量繁育种苗，供生产使用。以顶芽和茎段为材料，培养无菌苗，研究不同配比的激素对肉穗草生育的影 响，结果表明：在附加0，5mg／L BA和0．2mg／L NAA的MS培养基上诱导丛生芽，成活率达到41．67％；在 附加2．0rag／L BA+0．2mg／L NAA的MS培养基上增殖效果最好，增殖率达到7．47倍；在MS+0，lmg／L NAA+0．1mg／L IBA+活性碳0．2％培养基上生根率达100％。 关键词：肉穗草；组织培养；快速繁育 中图分类号：$567 文献识码：A Tissue Culture and Rapid Propagation of Sarcopyramis bodinieri Zhang Xuzhang，Zhou Yifei，Pan Da’ten (Crop College ofF~yian Agriculture and Forestry University,Fuzhou 350002) Abstract：Sarcopyramis bodinieri has the effect of cool antidote，and is widely used to treat hepatitis as a chinese herbal medicine．In southern area of Fujian has larger sales．But now，depletion of wildlife resources is increasing．Due to its low coefficient of natural reproduction and low survival rate of artificial cultivation division or cutting propagation，plant tissue cuhure is used for seed breeding for production use．In this paper the terminal buds and stems of Sarcopyramis bodinieri were used to breed no vaccine and study the effect of different hormones on growth and development．The survival rate of cluster shoots was 4 1．67％ on MS medi— am supplemented with 0．5 mg／L BA and 0．2 mg／L NAA．When cultured on media supplemented with 2．0 mg／ L BA and 0．2 mg／L NAA，the coefficient of multiplication was the best，about 7．47 times．Rooting of shoots was developed in MS medium supplemented with O．1 mg／L NAA．0．1 mg／L IBA and O．2％ activated charcoa1． Its rooting rate could reach 100％． Key words：Sarcopyramis bodini