烧伤患者创面金黄色葡萄球菌感染的耐药性与流行病学分析.pdfVIP

714 实用预防医学 2008年6月 第 15卷 第3期 Practical Preventive Medicine，Jun．2008，Vol 15，No．3 文章编号：1006—3110(2008)03—0714—02 烧伤患者创面金黄色葡萄球菌感染的 耐药性与流行病学分析 王勇军 ，周建党 ，曹 伟 摘要： 目的 了解烧伤患者创面感染金黄色葡萄球菌的耐药性及流行状况。 方法 收集烧伤病房2007年10 ～ 12月之间63例患者创面分离的金黄色葡萄球菌 63株，纸片扩散法检测其对10种抗生素的耐药率、随机引物扩增PCR (RAPD)检测其流行状况。 结果 63株金黄色葡萄球菌对氨苄西林的耐药率最高，没有检测到对万古霉素耐药的金黄 色葡萄球菌。56株金黄色葡萄球菌为耐甲氧西林的金黄色葡萄球菌(MRSA)，7株为甲氧西林敏感的葡萄球菌(MSSA)， 除万古霉素外，MRSA对其他9种抗生素的耐药率比MSSA高 16．1％～100％，63株金黄色葡萄球菌经RAPD分型分为7 个基因型，其中I、Ⅱ、Ⅲ、Ⅳ、VⅥ、Ⅶ型分别占12．7％、17．5％、9．5％、39．7％、9．5％、6．3％、4．8％。 结论 烧伤患者 创面金黄色葡萄球菌分离株对多种抗生素具有高耐药性，以MRSA感染为主，基因型以Ⅳ型为主。 关键词：烧伤刨面；金黄色葡萄球菌；耐药性；随机扩增多态性DNA 中图分类号：R644 文献标识码：A Resistance and Prevalence of Staphyl0c0ccus Aureus from Burn Wounds Infections WANG Yong—jun，ZH0U Jian—dang， CA0 wei(Department of Blood Transfusion，the Second Xiangya Hospital，Central South University，Changsha 410011， Hunan) Abstract： Objective To investigate the antibiotic resistance and prevalence of Staphylococcus aureu S(S．aureus) frOm burn wounds infections． Methods Sixty—three strains of S．aureus were COllected frOm C)ctober to December in 2007．The resistance rate was tested with routine Kjrby—Bauer method and the prevalence was detected by random amplified polymorphic DNA(RAPD)． Results The resistance rate to ampic…in 0f S．aureus was the highest；whichever of strains was resistant to vancomycin．Fifty six strains of S．aureus were methic…in resistant Staphylococcus aureus(MRSA)and sev— en strains were methic…in susceptible Staphylococcus aureus(MSSA)．Except for vancomycin，the resistance rates to other antibiotic of MRSA were higher from 16．1％ to 100％ than MSSA．Seven genotypes were separated by RAPD and the ratios Of l，ll，…，lV，V，Vl，and Vll were 12．7％ ，17．5％，9．5％ ，39．7％，9．5％，6．3％，and 4．8％，respectively． Conclu． si