外源钙离子对铅胁迫下小麦膜脂过氧化和根系活力的影响.PDF

安徽农业大学学报, 2017, 44(3): 508-512 Journal of Anhui Agricultural University [DOI] 10.13610/ki.1672-352x025 网络出版时间：2017/5/24 11:30:59 [URL] /kcms/detail/34.1162.S1130.050.html 外源钙离子对铅胁迫下小麦膜脂过氧化和根系活力的影响 * 赵腾飞，刘 颖，王尔美，王晴晴，代 莉，张云华 (安徽农业大学资源与环境学院，合肥 230036) 摘 要：为明确外源添加钙离子对铅污染区域小麦膜脂过氧化和根系活力的影响机理，利用1/2 Hoagland 营养 液培养新麦 26 ，研究外源添加不同浓度钙离子对铅胁迫下小麦幼苗叶绿素、抗氧化酶活性、丙二醛含量、细胞膜 透性及根系活力造成的影响。结果表明，外源钙的添加较有效地缓解铅胁迫对小麦的毒害，与Pb 处理相比，外源 钙离子处理叶绿素含量明显升高，Ca3 处理升高最多，达到 77.3% ；MDA 含量和细胞膜透性显著降低，Ca3 处理 分别下降29.9% 、24.5% ；抗氧化酶活性表现存在差异，Ca3 处理SOD、CAT 活性分别升高48.5% 、85.6%，而POD 活性降低26.9% ；Ca3 处理显著恢复了小麦根系活力，恢复率为56.2%。因此，试验表明以氧化钙调节pH 环境并 混合氯化钙的方法能一定程度缓解铅对小麦的毒害作用。 关键词：小麦；铅胁迫；外源钙；膜脂过氧化；根系活力 中图分类号：S512.1 文献标识码：A 文章编号：1672352X (2017)03050805 2+ Effects of exogenous Ca on membrane lipid peroxidation and root activity of wheat under lead stress ZHAO Tengfei, LIU Ying,WANG Ermei, WANG Qingqing, DAI Li, ZHANG Yunhua (School of Resources and Environment, Anhui Agricultural University, Hefei 230036) 2+ Abstract: In order to clarify mechanisms of exogenous Ca on membrane lipid peroxidation and root activity 2+ of wheat under Pb stress, effects of exogenous different concentrations Ca on chlorophyll content, antioxidant en- zyme activity, MDA content, cell membrane permeability and root activity in Xinmai 26 (grow in 1/2 Hoagland) seedlingunder Pb stress were studied. Results showed that the addition of exogenous calcium could alleviate the tox- icity of lead stress on wheat more effectively. Co