强脉冲光对红毛癣菌的体外抑制作用.PDFVIP

J South Med Univ, 2017, 37(6): 853-857 doi 10.3969/j.issn. 1673-4254.2017.06.25 ·853 · 基础研究 强脉冲光对强脉冲光对红毛癣菌的体外抑制作用红毛癣菌的体外抑制作用 黄 昊，汤红峰，陈 颖，郑秀芬，胡 勇，王瑞华，黄美玲 南方医科大学附属顺德第一人民医院皮肤科，广东 佛山 528300 摘要：目的探讨强脉冲光420 nm 滤光片在体外对红色毛癣菌的抑制作用及其机制。方法 实验所用红色毛癣菌落及菌悬液各 分为处理组（强脉冲光照射）和对照组（未照射），在照射前、照射后第2 天、第3 天，拍摄各组菌落，使用Photoshop cx7 软件以像素 计算菌落的面积观察菌落生长曲线变化，以及在照射6 h 后，通过噻唑蓝检测真菌菌悬液的活力；2 ，7-二氯荧光黄双乙酸盐荧光 探针检测各组真菌内活性氧变化，硫代巴比妥酸法检测各组真菌丙二醛含量改变。结果 IPL420 nm 对红色毛癣菌具有损伤作 用，其最适有效治疗参数为12 J/cm2 12脉冲，处理后6 h 后菌株活力下降约30%（P0.05），而处理后平板菌落停止生长，生长曲 线趋于直线；同时IPL 处理后真菌内活性氧水平升高（P0.05），伴随丙二醛含量上升（P0.05）。结论 IPL420 nm 可在体外通过 诱导红色毛癣菌内产生氧化应激，导致真菌损伤和死亡。 关键词：红色毛癣菌；强脉冲光；活性氧；氧化应激 Effect of intense pulsed light on Trichophyton rubrum growth in vitro HUANG Hao, TANG Hongfeng, CHEN Ying, ZHENG Xiufen, HU Yong, WANG Ruihua, HUANG Meiling Department of Dermatology, Shunde First Peoples Hospital Affiliated to Southern Medical University, Foshan 528300, China Abstract: Objective To investigate the inhibitory effect of 420 nm intense pulsed light on Trichophyton rubrum growth in vitro and explore the mechanism. Methods The fungal conidia were divided into treatment group with intense pulse light irradiation and control group without irradiation. The surface areas of the fungal colonies were photographed before irradiation and on the 2nd and 3rd days after irradiation to observe the changes in fungal growth. The viability of the fungus in suspension was detected at 6 h after irradiation using MTT assay. The intracellular reactive oxygen species (ROS) level in the fungus was determined using DCFH-DA fluorescent probe, and the MDA content was detected using TBA method. Results Intense pulse light (420 nm) irradiation caused obvious injuries in Trichophyton rubrum with the optimal effective light dose of 12 J/cm2 in 12 pulses. At 6 h after the irradiation, the fungus in suspension showed a 30% reduction of viability (P0.05), an