03-370 prot assay mech - Bio-Rad Laboratories（03 - 370 prot试验装置- Bio-Rad实验室）.pdf

protein assays tech note 1069 Colorimetric Protein Assays Introduction Bio-Rad offers four colorimetric assays for protein quantitation: the Quick Start™ Bradford protein assay, the Bio-Rad protein assay for general use, the DC™ protein Quick Start Bradford Bio-Rad RC DC ™ Protein Assay Protein Assay assay for samples solubilized in detergent, and the RC DC protein assay for samples in the presence of both reducing agents and detergents. The need for all types of assays is clear. The Quick Start Bradford protein assay comes with prediluted standards and 1x dye reagent for maximum convenience and ease of use. Both the Quick Start Bradford DC Protein Assay RC DC Protein Assay and Bio-Rad protein assays can be used to assay samples in common buffers, but are sensitive to many detergents Sedmak and Grossberg 1977). Bradford (1976) first present in concentrations greater than 0.1%; the DC protein demonstrated the usefulness of this principle in protein assay can be used to assay protein in the presence of 1% assays. Spector (1978) found that the extinction coefficient detergent in addition to many common reagents; the RC DC of a dye-albumin complex was constant over a 10-fold protein assay is both reducing agent compatible (RC) and concentration range. Thus, Beer’s Law may be applied for detergent compatible (DC). Consult Appendices A, B, C, accurate quantitation of protein by selecting an appr