鸭乙肝病毒人工感染雏鸭的试验研究.DOC

不同种雏鸭建立鸭乙肝病毒感染模型及抗病毒效果的实验研究 郝友华1，李安意2 ，丁红晖1 ，朱帆1 ，赵西平1 ，杨东亮1 (1. 华中科技大学同济医学院附属同济医院临床免疫研究室, 武汉 430030; 2. 华中科技大学同济医学院附属同济医院动物实验研究中心，武汉 430030 ) [摘要] 目的：探讨不同种雏鸭建立鸭乙肝病毒感染模型的影响因素，观察应用该模型抗病毒的效果。方法：采集鸭血清，应用PCR方法定性检测鸭血清中病毒DNA；定量PCR方法检测鸭血清中病毒DNA载量变化；用抗病毒药物处理，观察其在鸭DHBV感染模型中的抗病毒效果。结果：不同种鸭DHBV自然感染率不同，樱桃谷鸭为8.75%，湖北麻鸭两个批次分别为17.80%和10.68%；静脉注射和腹腔注射两途径均能致雏鸭感染DHBV，静脉注射感染率80%，腹腔注射感染率65%；鸭感染DHBV后，体内病毒载量维持在106~108copies/mL，可持续20d以上；抗病毒药物处理后，在不同DHBV模型中其抗病毒效果变化趋势一致。结论：鸭的种类和人工感染途径可影响DHBV感染率；雏鸭感染DHBV后其体内有持续性的病毒血症；DHBV感染模型是药物抗病毒研究较好模型。 [关键词] 鸭乙型肝炎病毒; 感染模型 [中图分类号xperimental Study on Duck Hepatitis B Virus Infection Model by Different Kinds of Ducklings and Anti-viral Effect Hao You-hua1, Li An-yi2, Ding Hong-hui1, Zhu Fang1, Zhao Xin-ping1, Yang Dong-liang1. (1. Division of Clinical Immunology，Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology，Wuhan 430030, China； 2. Center of Medical Laboratory Animal Science, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology，Wuhan 430030, China) [Abstract] Objective: To explore the factors in duck hepatitis B virus infection model by different kinds of ducklings and observe the antiviral effect in models. Methods: Collecting duck sera , qualitative detecting viral DNA by PCR method and detecting the changes of viral DNA load by the quantitative PCR method in duck sera. Observing the antiviral effects in duck DHBV infection models after antiviral drug treatment. Results: Natural infection rate of duck DHBV is different, Cherry Valley duck for 8.75%, two batches in Hubei sheldrake were 17.80% and 10.68% respectively. Both intravenous and intraperitoneal injection can cause ducklings infected with DHBV, infection rates of two ways were 80% and 65% respectiviely. Ducks infected with DHBV , the viral load is maintained at 106 ~ 108 copies / mL and persist for more than 20 days. Changing trends of antiviral effect in different DHBV models are coincident after treating by the a