2.4标准曲线的制备.PDFVIP

HPLC 测定硝酸舍他康唑尿素乳膏中硝酸舍他康唑的含量 秦荣新(广西柳州市人民医院药剂科，广西 柳州 545006) 摘要：目的 建立高效液相色谱法测定硝酸舍他康唑尿素乳膏中硝酸舍他康唑的含量。方法 色谱柱：Hypersil C18(4.6 mm 1 1 ×250 mm ，5 μm) ；流动相：乙腈-0.01 mol·L 磷酸氢二钠溶液(用稀磷酸调 pH=7.0)(37 ∶63) ；流速：1.0 mL·min ；检测 波长：260 nm 。结果 硝酸舍他康唑在 20.4~102.0 μg·mL1 内线性关系良好(r=0.999 3) ，平均回收率为 99.55% ， RSD=0.73%(n=6) 。结论 此法操作简便、结果准确、精密度好，可用于硝酸舍他康唑尿素乳膏的质量控制。 关键词：硝酸舍他康唑；硝酸舍康他唑尿素乳膏；高效液相色谱法；含量测定 中图分类号：R917.101 ；R917.795 文献标志码：B 文章编号：1007-7693(2010)09-0848-03 Determination of Sertaconazole Nitrate in Sertaconazole Nitrate and Urea Cream Formulation by HPLC QIN Rongxin(The People’s Hospital of Liuzhou, Liuzhou 545006, China) 作者简介：秦荣新，男，主管药师 Tel:E-mail:qinrongxin@163.com ·848 · Chin JMAP, 2010 September, Vol.27 No.9 中国现代应用药学2010 年 9 月第 27 卷第 9 期 ABSTRACT: OBJETCTIVE A high-performance liquid chromatographic method was developed for the determination of sertaconazole nitrate in sertaconazole nitrate and urea cream formulation. METHODS Hypersil C18 column (4.6 mm ×250 mm, 5 μm) was used along with a mobile phase consisting of acetonitrile-0.01 mol·L1 disodium hydrogen phosphate solution(37 ∶ 63), adjusted to pH 7.0 by phosphoric acid. The flow rate was 1.0 mL·min1, and the detection wavelength was 260 nm. RESULTS The detector response for sertaconazole nitrate is linear over the concentration range of 20.40102.0 μg·mL1, and the average recovery was 99.55%(RSD=0.73%, n=6). CONCLUSION The method is simple and accurate, it can be used for quality control of sertaconazole nitrate and urea cream formulation. KEY WORDS: sertaconazole nitrate; sertaconazole nitrate and urea cream; HPLC; content determination 硝酸舍他康唑是一种新型外用咪唑类抗真菌 醇稀释至刻度，摇匀，作为对照品溶