rneasy plus miniプロトコールとトラブルシューティング - qiagen.pdfVIP

September 20 10 ® RNeasy Plus Mini gDNA Eliminator RNA RNA 2 RNA 8 15 Sample Assay Technologies RNA RNA 100 ■■ RNA ■■ gDNA Eliminator DNA ■■ RNeasy RNA 100 µg RNA ■■ Buffer RLT Plus Buffer RLT Plus 1 x 107 RNA 6 ■■ COS RNA 10 35 µg RNARNeasy RNA 3 x 106 6 ■■ HeLa RNA 10 15 µg RNARNeasy RNA 7 x 106 6 ■■ NIH/3T3 RNA 10 10 µg RNA 1 x 107 Table 2 Handbook 13 RNA 3 4 x 106 RNA RNA DNA gDNA Eliminator RNA RNeasy Table 3 Handbook 14 HeLa 2 RNeasy Plus Mini 09/20 10 ■■ RNeasy Plus Mini Kit Important NotesHand- book 12 ■■ RNA Appendix A Handbook 34 ■■ –70 2 3 –70 37 RNA 3,000 5,000 x g 5 RNase 4 ■■ β- β-MEBuffer RLT Plus 1 ml Buffer RLT Plus 10 µl β-ME β-ME Buffer RLT Plus 15 25 1 ■■ Buffer RPE 4 96 100 ■■ Buffer RLT Plus ■■ Buffer RLT Plus Buffer RW 1 Safety information Handbook 6 ■■ ■■ 20 25 20 1. 1a 1b 7 1a. 1 x 10 300 x g 5 2 RNeasy Plus Mini 09/20 10