夹竹桃叶对蟾蜍离体坐骨神经干动作电位的影响 - 中国农学通报.pdfVIP

中国农学通报 2011,27(7):348-351 Chinese Agricultural Science Bulletin 夹竹桃叶对蟾蜍离体坐骨神经干动作电位的影响 1,2 1 向德标 ，伍松柏 1 （怀化学院生命科学系，湖南怀化418008 ； 2 怀化学院民族药用植物资源研究与利用湖南省重点实验室，湖南怀化418008 ） 摘 要：为了研究夹竹桃叶水提取物对蟾蜍离体坐骨神经动作电位的影响，将蟾蜍离体坐骨神经分为3 组，分别用10% 、20%和40%的夹竹桃水提取物对其进行浸泡，通过BL-420F 生物机能实验系统测定各 组离体坐骨神经干动作电位的振幅、传导速度、阈强度及不应期。结果显示经不同浓度夹竹桃水提取物 处理后，都可引起神经干动作电位振幅减小（P0.05 ），传导速度减慢（P0.05 ），不应期延长（P0.05 ），阈 强度增大（P0.05 ），并最终使坐骨神经动作电位消失，且动作电位均能恢复。试验结果表明夹竹桃叶水 提取物能可逆地阻滞神经动作电位的传导。 关键词：夹竹桃叶；蟾蜍坐骨神经干；动作电位；传导速度；不应期；阈强度 中图分类号：R285.5 文献标志码：A 论文编号：2010-3231 EffectsofNeriumindicumLeavesonCompoundActionPotentialofSciaticNervesofToad Xiang Debiao1,2, Wu Songbo1 1 ( LifeScienceDepartmentofHuaihuaUniversity, Huaihua Hunan 418008; 2KeyLaboratoryofHunanProvinceforStudyandUtilizationofEthnicMedicinalPlantResources, HuaihuaUniversity, Huaihua Hunan 418008) Abstract: To research the effects of the water extract from Nerium indicun leaves on action potential of the toad ’s sciatic nerves. The toad ’s sciatic nerves were divided into three groups, and infiltration with concentration of 10% , 20% and 40% of the water extract from Nerium indicum leaves. The amplitude, conduction velocity, threshold intensity and refractory period of action potentials of the sciatic nerves from each group were measured respectively by BL-420F organism function experiment system. The conclusion of study had indicated that the water extract fr