一株玉米秸秆纤维素分解菌株的分离鉴定及酶学性质.pdf

微生物学通报 May 20, 2016, 43(5): 991−997 Microbiology China /wswxtbcn tongbao@ DOI: 10.13344/j.microbiol.china.150863 研究报告 一株玉米秸秆纤维素分解菌株的分离鉴定及酶学性质 * 赵萍 夏文旭 郭健 雷晨瑶 王聪 肖宇轩 丁毛毛 王雅 ( 730050) 摘 要：【目的】筛选分离可以分解玉米秸秆纤维素的菌株。【方法】采用平板稀释法从土样中 分离纯化得到能够分解玉米秸秆纤维素的菌株，对分离得到的菌株进行生理生化及分子鉴定， 同时以菌株Penicillium spp. (CICC40361)作为对照，比较菌株纤维素酶和木质素酶的活性，研 究不同因素对菌株纤维素酶活力的影响，确定菌株纤维素酶动力学常数Km 值。【结果】分离得 到的菌株命名为 PL2# ，经生理生化及分子鉴定后，确定菌株 PL2#为羊毛状青霉(Penicillium lanosum) 。菌株PL2# 的纤维素酶和木质素酶活力高于对照菌株Penicillium spp. ；菌株PL2# 的 最优酶活测定条件为：1% CMC 底物浓度，pH 4.8 ，50 °C ，酶反应时间60 min 以及2 mL DNS 添加量。【结论】羊毛状青霉(Penicillium lanosum)菌株PL2# 比对照菌株Penicillium spp.具有更 好地降解玉米秸秆纤维素的性能。 关键词：菌株，玉米秸秆，纤维素，酶活 Isolation, identification of a strain decomposing corn stalk and characterization of its cellulose ZHAO Ping∗ XIA Wen-Xu GUO Jian LEI Chen-Yao WANG Cong XIAO Yu-Xuan DING Mao-Mao WANG Ya (Laboratory of Microbial Fermentation and Enzyme Technology, School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, Gansu 730050, China) Abstract: [Objective] The cellulose-decomposing strains for corn stalk were screened. [Methods] Agar dilution method was used to screen strains decomposing corn stalks from soil samples. Strains were identified by their morphological, physiological and biochemical properties and internal transcribed spacer sequence analysis. Then we compared the activities of cellulose and lignin degrading enzyme of the isolated strains with that of Penici