磷对小麦细胞镉、锌的积累及在亚细胞内分布的影响 - 环境科学学报.pdf

第 19 卷第 6 期 环 　境 　科 　学 　学 　报 Vol . 19 ,No . 6 1999 年 11 月 AC TA SCIEN T IA E CIRCU M STAN T IA E Nove . ,1999 简 　报 　磷对小麦细胞镉、锌的积累及在亚细胞内分布的影响 (南京农业大学理学院应用化学系 ,南京 　2 10095) 杨志敏 　郑绍建 　赵秀兰 　胡霭堂　 μ - 1 ( - 1) 摘要 　研究了小麦细胞在含有 0 ,50 mol ·L 镉的 M S 培养液中,提高磷浓度 0 5 , 15 ,3 5 mmol ·L 对细胞内磷 、镉 、锌 浓度及磷 、镉 、锌在细胞壁 、液泡 、细胞质内含量分布的影响. 提高介质供磷水平促进了细胞的生长 ,但降低镉 、锌两种元素在 细胞中的含量. 增磷还能降低镉和锌在细胞壁 、液泡和细胞质中含量. 结果表明,较高浓度的磷和镉分布于细胞壁. 小麦细胞 的液泡也积累了一定量的镉. 锌在液泡中的浓度大于其在细胞质和细胞壁中的浓度. 上述结果表明,磷对增强小麦细胞的耐 镉性具有重要的作用. 关键词 　磷 ;镉 ;锌 ;细胞 ;含量. Eff ect of phosphorus on the accumulation of cadmium and zinc in sus pensioncultured wheat cell s ( YAN G Zhimin ,ZHEN G Shaojian ,ZHAO Xiulan , HU Ait ang 　 Depart ment of Applied Chemistry , College of Sci ences ,Nanjing Agricultural Universit y ,Nanjing 2 10095) Abstract 　Suspensioncultured wheat cells were exposed to t hree levels of phosphorus ( 05 , 15 , 35 - 1) ( μ - 1) mmol ·L and t wo levels of cadmium 0 ,50 mol ·L wit h a 7day period , and t he concent ration of phosphorus ,cadmium and zinc in t he cells , cell wall ,vacuoles and cytoplasm were examined . As t he phos phorus content increased t he upt ake of cadmium and zinc decreased . However ,an increase of cells growt h was observed . The content of cadmium and zinc in cell wall ,cytoplasm and vacuoles also decreased wit h t he increase of phosphorus. The result s suggested t hat most of P and Cd was associated wit h cell wall , and zinc was accumulated mainly in t he vacuoles. Keywords 　phospho