利用四氧嘧啶建立糖尿病大鼠模型研究.docVIP

利用四氧嘧啶建立糖尿病大鼠模型的研究 刘燕，黄登峰，王静，彭飞，张文博，李扬 【摘要】目的：本实验研究四氧嘧啶不同给药途径对大鼠糖尿病模型造模成功的概率。方法：不同给药途径分为腹腔注射组和静脉注射组。以大鼠为实验对象，首先利用四氧嘧啶(Alloxan,AXN)进行不同途径造模，造模后断尾取血并采用邻甲苯胺法测定血糖,根据血糖值高低， 从而判断造模成功与否。结果：初步结果表明腹腔注射组造模成功率大于静脉注射组。但是几天后，大鼠有不同程度的死亡现象，是因为四氧嘧啶的注射量还是其他原因，这个问题有待进一步探讨，研究。结论：造模成功，从造模稳定性看，腹腔注射成模率高死亡率低、模型较稳定，为最佳给药途径。 【关键词】：四氧嘧啶；血糖；给药途径 AXN’s different routes of administration on the research of the pattern of diabetes mellitus mice (LIU Yan,HUANG Deng-feng,WANG Jing ,PENG Fei,ZHANG Wen-bo,LI Yang) (Department of Biology,BengBu Medical College, Bengbu 233030, Anhui, China) Abstract:Objectives:Comparing AXN ‘S different routes of administration on the research of the pattern of diabetes mellitus mice ,we could get the probability of success. Different routes of administration included the groups of intraperitoneal injection and intravenous injection.We took mice as experiment objects .Method:First we used AXN ‘S different routes of administration to get the pattern .Then we used the adjacent toluidine method to measure blood sugar level. On the basis of the level of blood sugar,we could know the probability of success. Result: As a result,the probability of success by intraperitoneal injection was higher than intravenous injection. But few days later,mice had different levels of dead phenomena.We had to go futher on the research of the question which was due to the AXN or other reasons . In conclution ,We succeed in getting the pattern .We took stability into account .We found that the group by intraperitoneal injection had more possibility to getting the pattern ,lower death rate and better stability.According to these , intraperitoneal injection was the best route of administration. Key words: AXN; blood sugar; diffrerent routes of administration 由于四氧嘧啶诱导的大鼠糖尿病模型与人类Ⅰ型即胰岛素依赖性糖尿病类似，故四氧嘧啶是制备大鼠糖尿病模型的常用药物。其作用机制是通过产生超氧自由基选择性地破坏胰岛B 细胞,使细胞内DNA 损伤,同时激活多聚ADP 核糖体合成酶的活性,从而使辅酶Ⅰ含量下降,两者共同作用导致mRNA 功能受损,引起细胞死亡，导致血中胰岛素浓度下降及高血糖产生，形成胰岛素依赖性糖尿病【1】。据文献报道AXN 致胰岛B细胞损伤与几种因素有关, 其中 AXN 使B细胞内”钙超载”