深海鲑鱼皮来源ACE抑制肽的分离及鉴定.pdfVIP

现代食品科技 Modern Food Science and Technology 2016, Vol.32, No.6 深海鲑鱼皮来源ACE 抑制肽的分离及鉴定 刘文颖，林峰，金振涛，陈亮，谷瑞增，鲁军，蔡木易 （中国食品发酵工业研究院，北京市蛋白功能肽工程技术研究中心，北京 100015） 摘要：为了考察海洋胶原低聚肽中ACE 抑制肽的活性和结构，本文以深海鲑鱼皮为原料，采用两步酶解法制备出海洋胶原低聚 肽，在对其分子量分布进行分析的基础上，测定了其血管紧张素转化酶（ACE ）抑制活性。结果表明，海洋胶原低聚肽中分子量小于 1000 u 的组分高达90.79%，主要分布在132~576 u 范围内，其ACE 抑制活性的IC50 为1.18±0.12 mg/mL。然后利用反相高效液相色 谱（RP-HPLC ）对海洋胶原低聚肽进行分离纯化，收集11 个组分峰，对其ACE 抑制活性进行了测定。结果表明，有7 个组分的ACE 抑制活性比海洋胶原低聚肽高。最后利用Q-TOF 质谱仪对收集的11 个组分进行了结构鉴定，并对鉴定出的15 个肽段进行了ACE 抑 制活性测定。结果表明，15 个肽段均有一定的ACE 抑制活性，其中Ala-Pro （AP ）、Val-Arg （VR ）、Gly-Arg （GR ）的ACE 抑制率 比海洋胶原低聚肽高，IC50 值分别为0.07±0.01 mg/mL、0.35±0.03 mg/mL、0.92±0.85 mg/mL ，活性大约是海洋胶原低聚肽的16.86、 3.37、1.28 倍，是具有较高ACE 抑制活性的肽段。 关键词：海洋胶原低聚肽；ACE 抑制肽；分子量分布；分离纯化；结构鉴定 文章篇号：1673-9078(2016)6-170-176 DOI: 10.13982/j.mfst.1673-9078.2016.6.027 Separation and Identification of ACE Inhibitory Peptides Derived from Deep-Sea Salmon Skin LIU Wen-ying, LIN Feng, JIN Zhen-tao, CHEN Liang, GU Rui-zeng, LU Jun, CAI Mu-yi (Beijing Engineering Research Center of Protein and Functional Peptides, China National Research Institute of Food and Fermentation Industries, Beijing 100015, China) Abstract: To investigate the activity and structure of angiotensin-converting enzyme (ACE) inhibitory peptides derived from marine collagen oligopeptides (MCOPs), MCOPs were prepared from deep-sea salmon skin by two-step enzymolysis. Based on analysis of the molecular weight distribution, the ACE inhibitory activity of MCOP was determined. The results showed that the components with a molecular weight of less than 1000 u in MCOP accounted for up to 90.79% of all proteins and were mainly distributed in the range of 132~5