靛蓝及其同类色素的微生物生产与转化-微生物所期刊联合编辑部.PDF

生 物 工 程 学 报 Chin J Biotech 2008, June 25; 24(6): 921-926 journals.im.ac.cn Chinese Journal of Biotechnology ISSN 1000-3061 cjb@im.ac.cn © 2008 Institute of Microbiology, CAS CSM, All rights reserved 综 述 靛蓝及其同类色素的微生物生产与转化 韩晓红, 王伟, 肖兴国 中国农业大学生物学院植物生理学与生物化学国家重点实验室, 北京, 100094 摘 要: 靛蓝类色素广泛应用于印染、食品和医药工业, 其环境友好的合成或生产途径越来越受到人们的关注, 特别是 微生物生物合成。已经鉴定和分离了能够合成靛蓝类色素的多种微生物, 并且明确了起催化作用的主要是单加氧酶和双 加氧酶。已经克隆和利用了一些加氧酶的基因, 构建了工程菌, 优化了其发酵过程。同时, 微生物合成靛蓝的生物转化 也已经起步。这些进展将带来环境友好的靛蓝类色素的合成与生产。 关键词: 靛蓝, 靛玉红, 微生物合成, 生物转化 Microbial Biosynthesis and Biotransformation of Indigo and Indigo-like Pigments Xiaohong Han, Wei Wang, and Xingguo Xiao State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094, China Abstract: Indigo and indigo-like pigments are widely used in the industry of textile, food and medicine. Now people pays more and more attention to developing an alternative method of indigo production which is “environment-friendy”, especially microbial biosynthesis of indigo. Many microorganisms involved in the biosynthesis of indigo have been isolated and characterized, and monooxygenase and dioxygenase have been identified to catalyze indigo biosynthesis. Some genes encoding for these enzymes have been cloned and used to construct “engineering bacteria”. With this kind of bacteria, more efficient fermentation systems for indigo production have been exploited. In the meantime, biotransformation