利福平醇溶说明书web-中科瑞泰.PDF

中科瑞泰 （北京）生物科技有限公司 Tel: 01082598075 Fax: 010 http:// E-mail: real-times@163.com Rifampicin(醇溶) 利福平 C H N O MW 822.94 CAS Number: 13292-46-1 43 58 4 12 ● Storage：4 C ● Specification： Assay……………≥97% (HPLC) Form……………orange-brown to red-brown powder Solubility…………DMSO: 100 mg/ml H O: 2.5 mg/ml at 25 C pH 7.3 2 ● Product description： Rif is highly active against Gram-positive bacteria, such as staphylococci, streptococci, pneumococci but is less active against Gram-negative organisms. Rif has activity against a wide range of microorganisms such as mycobacteria including Mycobacterium tuberculosis and M. leprae. The minimum inhibitory concentrations (MIC) for the most sensitive microorganisms (chlamydia, staphylococci) are in the range of about 0.01-0.02 µg/ml. The inhibitory activity of Rif remained practically unchanged between pH 5.5-8.0. Rif inhibits bacterial DNA-dependent RNA polymerase (the enzyme responsible for DNA transcription) by forming a stable enzyme-drug complex with the ß-subunit of RNA polymerase (RNAP-Rif), rpoB gene (binding constant of 10-9 M at 37 C). Rif suppresses the initiation of chain formation (but not chain elongation) in RNA synthesis. There is some inhibition of mammalian RNA polymerases at much higher concentrations of Rif than that for bacterial RNA polymerases. Rif has also antifungal activity probably due to some other mechanism of action than inhibition of a fungal RNA polymerase. ● 利福平母液配制方法： （注：利福平比较难溶解，有多种溶解方法，以下方法请参考） 1. 10mg/ml 利福平-甲醇溶液，配制量：10ml 【推荐方法】 取100mg 利福平迅速加到15ml 离心管中，加入7-8ml 甲醇溶解，混匀彻底溶解后补加甲 醇至总体积10ml；0.22