HPLC同时测定宣肺止咳合剂3种指标成分的含量.PDF

HPLC 同时测定宣肺止咳合剂 3 种指标成分的含量 * 王莹，杜静 (杭州市红十字会医院，杭州 310003) 摘要：目的 建立反相高效液相色谱法同时测定宣肺止咳合剂中葛根素、大豆苷、甘草酸的含量。方法 采用 Agilent Zorbax SB-C18(4.6 mm×250 mm ，5 μm) 色谱柱，检测波长：254 nm ，以乙腈-0.1% 甲酸水为流动相，梯度洗脱，柱温为 30 ℃。结果 葛根素、大豆苷、甘草酸浓度分别在 99.14~991.4 ，22.50~225.0 ，22.60~226.0 µg·mL1 内与峰面积呈良好 的线性关系(r ≥0.999 8) ，平均回收率分别为 99.2% ，98.8% ，99.3%。结论 该方法简便、准确、重复性好，为宣肺止咳 合剂的质量评价与质量控制提供了科学依据。 关键词：宣肺止咳合剂；高效液相色谱法；葛根素；大豆苷；甘草酸；含量测定 中图分类号：R284.1；R917.101 文献标志码：B 文章编号：1007-7693(2016)05-0599-03 DOI: 10.13748/ki.issn1007-7693.2016.05.018 Study on Simultaneous Determination of Three Main Marker Components in Xuanfeizhike Mixture by HPLC * WANG Ying, DU Jing (Hangzhou Red Cross Hospital, Hangzhou 310003, China) ABSTRACT: OBJECTIVE To establish an HPLC method for simultaneous determination of puerarin, daidzein and glycyrrhizic acid in Xuanfeizhike mixture. METHODS Isolation of sample was achieved on an Agilent Zorbax SB-C18(4.6 mm× 250 mm, 5 μm), under the 254 nm detection wavelength, gradient eluted by acetonitrile-0.1% formic acid, and the column temperature was maintained at 30 ℃. RESULTS The linearity ranges of puerarin, daidzein and glycyrrhizic acid were 99.14991.4, 22.50225.0, 22.60226.0 µg·mL1, respectively(r ≥0.999 8). The average recoveries(n=9) of them were 99.2%, 98.9%, 99.3%. CONCLUSION The method is simple, accurate and has a good repeatability. The study provides scientific foundation for quality evaluation and quality control standards for Xuanfeizhike mixture. KEY WORDS: Xuanfeizhike mixture; HPLC; puerarin; daidzein; glycyrrhizic acid; content determination 宣肺止咳合剂(原名清肺饮) 由麻黄、杏仁、生 具有抗炎、抗氧化应激、抗过敏、改善血液循环 石膏、葛根、甘草、僵蚕、知母等组成，是笔者