基金项目：国家现代产业技术体系“国家现代产业技术体系（蛋鸡）专项 .DOCVIP

基金项目：国家现代产业技术体系“国家现代产业技术体系（蛋鸡）专项经费”(CARS-41-K19)。 第一作者简介：陈丽园，女，1981年出生，福建永定人，助理研究员，硕士，主要从事饲料及环境微生物方面的研究。E-mail：semidry2@163.com。 通信作者：詹凯，男，1968年出生，安徽安庆人，研究员，博士，从事家禽育种及生产研究。通信地址：230031 安徽省合肥市农科南路40号省农科院创新大楼402室 安徽省农科院畜牧所，Tel：0551E-mail：zhankai633@126.com。 收稿日期：2014-02-20，修回日期：2014-05 土传病害生防菌的分离筛选及鉴定 陈丽园1，詹 凯1，鲁恒星1，戚仁德2 （1安徽省农业科学研究院畜牧兽医研究所，合肥 230031；2安徽省农业科学院植物保护研究所，合肥 230031） 摘 要：为了使蛋鸡粪有机肥兼有防治作物土传病害的多功能性。本实验从安徽省农科院实验田及蛋鸡养殖场等环境中共分离出170株真菌，首先将分离菌与辣椒炭疽病(Colletotrichum capsici)进行平板对峙实验，再通过复筛选出7株菌，同时对该7株菌进行抑菌谱、耐温实验和蛋鸡粪肥中增殖实验，效果较好的菌株最终进行分子生物学鉴定。结果表明：筛选出的菌株中编号为TCC53对油菜菌核病和麦纹枯的抑菌率分别可达90.4%、82.3%，TCC157对辣椒炭疽病和辣椒疫霉的抑菌率分别可达88.6%、71.2%，同时该两株菌均可在40℃以上生长。通过分子生物学鉴定得出TCC53和TCC157分别为棘孢曲霉(Aspergillus aculeatus)和皮落青霉(Penicillium crustosum)。皮落青霉和棘孢曲霉均为环境中常见真菌，生长快、不具有致病性， 关键词：蛋鸡粪；生防有机肥；棘孢曲霉；皮落青霉 中图分类号：Q89 文献标志码：A 论文编号：2014-0392 Screening and Identification of Antagonisms Against the Soilborne Pathogens Chen Liyuan1, Zhan Kai1, LU Henxing1, Qi Rende2 (1Institute of Animal Husbandry and Veterinary, Anhui Academy of Agricultural Sciences, Hefei 230031; 2Institute of Plant Protection, Anhui Academy of Agricultural Sciences, Hefei 230031) Abstract: To make the laying hen manure have the multi-function of preventing soil-borne diseases. We isolated 170 strains from the experimental field and laying hens farms of Anhui Academy of Agricultural. 7 strains were screened out by the plate confrontation experiment carried out to test the inhibition effect of 170 strains to Colletotrichum capsici. After that, we carried out inhibitory spectrum experiments, temperature experiments and proliferation assays with these 7 strains to screen out the effective strains. At last, better ones were taken to molecular biological identification test. The results showed that the inhibitory rates of TCC53 to Sclerotinia sclerotiorum and Rhizoctonia cerealis were 90.4% and 82.3% respectively, the inhibitory rate of TCC157 to Colletotrichum capsici and Phytophthora capsici were 88.6% and 71.2%. And both