延迟增强核磁共振评估经皮球囊封堵冠状动脉法制备的小型猪心增强 .PDFVIP

·34 · doi 10.3969/j.issn. 1673-4254.2013.01.08 J South Med Univ, 2013, 33(1): 34-39 基础研究 延迟延迟增强核磁共振评估经皮球囊封堵冠状动脉法制备的小型猪心增强核磁共振评估经皮球囊封堵冠状动脉法制备的小型猪心 肌梗死肌梗死模型模型 1 2 3 4 尹巧香 ，赵玉生 ，王 姮 ，裴志勇 1 3 2 4 空军总医院 干部病房心内科，神经内科，北京 100142；解放军总医院老年心血管病研究所，北京 100853；北 京军区总医院干部病房一科，北京 100700 摘要：目的 利用延迟增强核磁共振（DE-MRI）评估经皮球囊封堵冠状动脉法制备小型猪心肌梗死模型。方法 麻醉后，经小型 猪右侧股动脉置入2.0 mm× 15.0 mm 球囊至左前降支（LAD)中远段，完全封堵血流90 min。DE-MRI 评估心梗后梗死面积及心 功能变化，病理检查进一步明确结果。结果 DE-MRI 显示平均心肌梗死面积为10.2±2.9 cm3 。与对照组比较，急性心肌梗死组 心输出量、左室射血分数和心搏量均明显下降（P0.001）；而左室舒张末容积和左室收缩末容积均升高（P0.05）。DE-MRI 结果 和左室短轴病理切片相对应。结论 建立了有效的评估小型猪急性心肌梗死模型，适用于评价干细胞移植治疗急性心肌梗死。 关键词：急性心肌梗死；延迟增强核磁共振；球囊封堵；小型猪 Delayed-enhanced magnetic resonance imaging for assessing a minipig myocardial infarction model established by percutaneous balloon occlusion 1 2 3 4 YIN Qiaoxiang , ZHAO Yusheng , WANG Heng , PEI Zhiyong 1Department of Elderly Cardiology, 3Department of Neurology, General Hospital of Air Force, Beijing 100142, China; 2Institute of Geriatric Cardiology, General Hospital of PLA, Beijing 100853, China; 4Department of Elderly Cardiology, General Hospital of Beijing Military Command, Beijing 100700, China Abstract: Objective To assess a minipig model of acute myocardial infarction (AMI) established by percutaneous balloon occlusion using delayed-enhanced magnetic resonance imaging (DE-MRI). Methods A minipig model of AMI was established by placement of a 2.0 mm × 15.0 mm percutaneous transluminal coronary angioplasty balloon in the middle left anterior descending artery (LAD) through a percutaneous femoral puncture in the right inguinal region. The left anterior descending coronary artery