卷叶蛾赤眼蜂体内共生菌Wolbachia与其产雌孤雌生殖行为关系的研究.pdf

___________________________________________________________________________ 卷叶蛾赤眼蜂体内共生菌Wolbachia 与其产雌 孤雌生殖行为关系的研究* 1 1** 2 3 项宇 沈佐锐 黄大庄 李瑞军 （1.中国农业大学农学与生物技术学院 北京 100094；2.河北农业大学森保学院 保定 071000;3.河北农业大学植保学院 保定 071000） Relationship Between Thelytokous Character of Trichogramma cacoeciae and Its 1 1 2 3 Symbionts Wolbachia. XIANG Yu , SHEN Zuo-Rui , HUANG Da-ZHuang ,LI-Ruijun (1.Department of Entomology, Chinese Agriculture University, Beijin g, 100094; 2.College of Forest Protection, Hebei Agriculture University, Baoding, 071000; 3.College of Plant Protection, Hebei Agriculture University, Baoding, 071000 ) Abstract: Based on both molecular detection of Wolbachia wsp gene and biological character observation, relationship between thelytokous character of Trichogramma cacoeciae and its symboint Wolbachia has been studied under the condition of 30℃ of 10 generations. And due to continuous heat treatment thelytokous character of T. cacoeciae was altered and PCR result of Wolbachia wsp gene was negative after 4 generations. It shows that there is correlation between thelytokous character of T. cacoeciae and Wolbachia to certain extend. Keywords: Thelytoky, Wolbachia, Molecular detection, high temperature, Trichogramma cacoeciae 摘要：在 30℃的条件下，连续饲养卷叶蛾赤眼蜂 10 代，观察每代蜂的生物学特 性，并进行 Wolbachia 的wsp 基因的分子检测，从分子生物学和传统生物学的 双重角度研究卷叶蛾赤眼蜂产雌孤雌生殖行为与其体内共生菌 Wolbachia 的关 系。结果表明，高温下卷叶蛾赤眼蜂体内的 Wolbachia 被抑制，4 代以后 wsp 基 因的PCR 检测结果变为阴性；同时赤眼蜂的产雌孤雌生殖行为的改变程度也随