nm23-H1及NGAL在宫颈癌中表达及临床研究.doc

nm23-H1及NGAL在宫颈癌中表达及临床研究[摘要] 目的 探讨nm23-H1、NGAL在宫颈癌中的表达与预后的关系。方法 应用免疫组化SABC法检测100例宫颈癌中nm23-H1、NGAL的表达。结果 宫颈癌中nm23-H1、NGAL的阳性率分别为48%和61%。其中，nm23-H1在不同年龄分组、临床分期和病例类型中阳性表达率差异无统计学意义（P＞0.05）；在有肿瘤转移的标本中阳性率为67.86%，低分化标本中阳性率为76.19%，肿瘤直径≥4cm标本中阳性率表达率56.52%，差异有统计学意义（P＜0.05）。NGAL在不同临床分期和不同肿瘤直径组中阳性表达率无统计学意义（P＞0.05）；＞45岁组标本中阳性率为70.49%，在鳞癌标本中阳性率为70.00%，在发生肿瘤转移的标本中阳性率为82.14%，在中高分化标本中阳性率为67.09%，差异有统计学意义（P＜0.05）。结论 nm23-H1和NGAL的表达情况对宫颈癌的预后评估有一定的意义。 [关键词] 宫颈肿瘤；基因表达；免疫组化 [中图分类号] R737.33[文献标识码] A [文章编号] 1673-9701（2010）19-01-03 Expression of nm23-H1 and NGAL Gene in Cervical Cancer CHENG Xiaoyan1ZHENG Hongfeng1LV Weichao1SONG Yun2 1.Department of Obstetrics and Gynecology，The Sixth Affiliated Hospital of Wenzhou Medical College The People’s Hospital of Lishui City，Zhejiang Province，Lishui 323000，China；2.Department of Pathology，The Sixth Affiliated Hospital of Wenzhou Medical College The People’s Hospital of Lishui City，Zhejiang Province，Lishui 323000，China [Abstract] Objective To investigate the expression of nm23-H1 and NGAL gene in cervical cancer and its effect on the development of the cervical cancer. Methods The expression of nm23-H1，NGAL gene in 100 cases cervical cancer were studied with SABC immunohistochemcal technique. Results In cervical cancer，the positive expression rate of nm23-H1 and NGAL were 48% and 61%. The positive expression rate of nm23-H1 have no differences in different age groups，clinical stage and type of cases（P＞0.05）. The positive expression rate of nm23-H1 in the tumor metastasis，poor differentiation and tumor diameter ≥ 4cm specimens are high positive expression rate，respectively，67.86%，76.19% and 56.52%，the difference in their respective groups was significant（P＜0.05）. There were no differences in positive expression rate in different clinical stages and different tumor size groups of NGAL（P＞0.05）. NGAL have more positive expression rate in ＞45-year-old group，squamous cell carcinoma，tumor metastasis，high differentiation and tumor diameter