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史密斯桉组织培养技术研究
27 2 Vol. 27 No. 2
20 10 12 EUCALYPT SCIENCE TECHNOLOGY Dec. 2010
管朝旭, 黄 键, 曾德贤, 朱仁刚, 范林元, 陈 英
( , 650215
: , H Cl2 1% ,
g
, 12. 5% ; , F+ 6- BA 0. 5 mg L - 1+ IBA 0. 3
mg L - 1 , , 3. 7; ,
H+ 6- BA 0. 125~ 0. 25 mg L - 1+ IBA 0. 25 mg L - 1+ VC 20 mg L - 1+ VB2 10 mg L - 1 ,
, ; 1/ 3 MS , 0. 25 mg L - 1 IBA
, 37. 8%
: ; ;
: S722. 3+ 7 : A
Study on Tissue Culture of Eucalyp tus smithii
GUAN Zhao- xu, HUANG Jian , ZENG De- xian, ZHU Ren- gang, FAN Lin- yuan, CHEN Jian- ying
( Forestry Seed and Seedling Station of Yunnanp rovince , K unming 650215, Yunna n, China
Abstract: The technology of E ucalyp tus smithii tissue culture was discussed . The experiment showed that the
2 2
treatment effective to prevent browning was with dipping them into 1% CaCl after treated with HgCl .
- 1
The rate of brown and mortality rate was only 12. 5% . The medium of F+ 6- BA 0. 5 mg L + IBA 0. 3
- 1
mg L could promote the induction of adventitious buds and multiplication in the initial budding culture
and the reliminar sta e of subcultures , the ratio of multi lication could reach 3. 7. A lar e ratio could ain
p y g p g g
and elon ation rowth of buds could be stimulated b us
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