鸡胸腺肽的提取及其生物学活性的初步研究.pdfVIP

⑩中同采禽2008年第30卷第4期 China Poultry Vo1．30,No．4．2008 鸡胸腺肽的提取及其生物学活性的初步研究 郭设平 ，王沂蒙 ，姜玉坤 ，孙宏鑫 ，李明峰 ，齐旭阳 ，庄国宏 ，江国托 (1．大连三仪生物工程研究所，辽宁大连 116036； 2．大连工业大学生物与食品工程学院，辽宁大连 116034) 摘 要：采用两种不同的方法提取鸡胸腺肽，计算胸腺肽得率，鉴定和比较提取物的 生物学活性。结果显示，两种方法的提取物在体外不仅能使脱E受体的淋巴细胞恢复其E 受体的功能，并能促进体外培养的淋巴细胞增殖；而且能增强鸡的淋巴细胞增殖能力，增 加鸡外周血中 一干扰素的含量。除了E玫瑰花环实验，各项体内外的活性检测均显示第二 批鸡胸腺肽具有较高的活性，并且与购买的人用胸腺肽相当。本研究的结果表明，提取方 法二操作简便，产率高，其提取物生物学活性较高，可用于兽医临床研究。 关键词：胸腺肽；提取；生物学活性 Preminary Study on Extraction of Chicken Thymosin and its Biological Functions GUO Sheping ，WANG Yimengl,2,JIANG Yukun ，SUN Hongxin1,2 LI Mingfengl,2,QI Xuyang ， ZHUANG Guohong ．JIANG Guotuo ’ (1．Dalian Sanyi Bioengineering Institute，Dalian，Liaoning 1 1 6036； 2．Bio—food Technology Faculty，Dalian Polytechnic University，Dalian，Liaoning 1 16034) Abstract：With the purpose of developing a simple and fast method for extracting thymosin,two methods were tested． The harvest of them were calculated．Further，the biological function of the extraction was studied．The results showed that thymosin obtained by both methods could help the inactivated E receptors recover．It also could stimulate lymphocytes proliferation in vitro．Animal teas showed that thymosin enhanced lymphocytes proliferation instimulated by ConA，and it also could induce IFN-T secreted into peripheral blood．Thymosin purified by the second method showed better biological function and equal to the purchased thymosin in all tests except E rosette assay．The results suggested that the second method had more application potential，thymosin obtained by this method showed fine biological function and suitable to use in veterinary clinic． Key words：thymosin；extraction；biological functions 胸腺肽是一组由胸腺上皮细胞分泌的多肽类 作为一种免疫调节剂，已广泛用于医学临床。在 激素。它能够调节人和动物的