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大鼠外核苷酸焦磷酸酶
武汉基因美科技有限公司
Rat Ectonucleotide
Pyrophosphatase/Phosphodiesterase
2 (ENPP2)ELISA Kit
CatalogueNumber:JYM0711Ra
Forresearchuseonly.
For the quantitative in vitro determination of ENPP2
concentrations in Rat serum, plasma, culture media or any
biological fluid.
Expirationdate:sixmonths.
Storage 2-8 .
: ℃
Principle
ThisELISA kit uses Sandwich-ELISA asthemethod.TheMicroelisa stripplateprovided
in this kit has been pre-coated with an antibody specific to ENPP2. Standards or samples are
added to the appropriate Microelisa stripplate wells and combined to the specific antibody.
Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for ENPP2 is added to
each Microelisa stripplate well and incubated. Free components are washed away. The TMB
substrate solution is added to each well. Only those wells that contain ENPP2 and HRP
conjugated ENPP2 antibody will appear blue in color and then turn yellow after the addition
of the stop solution. The optical density (OD) is measured spectrophotometrically at a
wavelength of450nm.The OD value isproportional tothe concentration ofENPP2.You can
calculate the concentration of ENPP2 in the samples by comparing the OD of the samples to
thestandard curve.
地址:武汉市洪山区高新大道666号光谷生物城 电话:027
1
网址: E-mail:jiyinmei027@163.com
武汉基因美科技有限公司
Materialsprovidedwiththekit
Materialsprovidedwith
48determinations 96determinations Storage
thekit
1 User manual 1 1 R.T.
Closureplate
2 2 2 R.T.
membrane
3 Sea
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