LTQOrbitrap常见问题—仪器校正.pptVIP

* * * * * * * * * * * * * * * * * * When the polyimide coating on the outside of the fused silica of the sample tube elongates, the sample does not come in contact with the ESI needle and sensitivity is decreased. It is good practice to cut the fused silica on a regular basis to minimize problems associated with the elongation of the polyimide coating. * The elongation of the coated fused silica sample tube leads to (1) spray distortion, by altering the shape of the emerging mist of droplets, and (2) decreased ionization/transfer efficiency by preventing the direct contact between the flow of sample and the high voltage in the electrospray needle. Ions (protonated or deprotonated molecules, according to the sign of the applied high voltage) are still observed, though at a much lower level, due to secondary field desorption/ionization mechanisms. * * * * * * * * * * * * * * * * LTQ Orbitrap常见问题—仪器校正 如果检查ESI参数后喷雾的结果还是不稳定 检查： 校正液管路是否有漏液。 校正液管路各接头连接是否有死体积。 关闭扫描，检查probe的液滴是否均匀地流出。 检查ESI探针内石英毛细管的安装是否正确 检查ESI探针内石英毛细管的棕色涂层是否有延长现象，如果有，一定要将之切割使石英毛细管末端平整。 使用金属针或者HESI，检查金属针的尖端是否有弯折现象。 石英毛细管聚合物的延长是毛细管在电场的作用下吸收了某些溶剂，特别是乙腈而造成的 为确保最佳的喷雾效果石英毛细管必须切割平整，并安装于ESI套针内1mm Sheath Liquid ESI Needle ESI Needle Sample Polyimide Polyimide Fused Silica Fused Silica ESI Needle ESI Needle ESI Needle Sample Polyimide Polyimide Fused Silica Fused Silica ESI Needle ESI Needle Sheath Liquid Sheath Liquid Sheath Liquid ESI Needle 石英毛细管聚合物的延长以及石英毛细管的安装 石英毛细管聚合物的延长 LTQ Orbitrap校正的其他注意事项 1.当且只有当喷雾（信号）稳定的情况下才去运行FT的以下两项校正： Storage transmission calibration FT transmission calibration 所以如果IT值过高，校正前请先清洗仪器，令仪器的IT值尽量符合前面所说的条件，即： NL值（in Centroid） IT值（ms） LTQ Orbitrap Velos： E7级别 ＜0.1 非LTQ Orbitrap Velos： E6级别 ＜1.0 2. 注意负离子校正与正离子校正的参数设定有所不同，总结来说负离子与正离子相比：气流大，流速高，电压低。具体请看前面。 3.当长时间只使