IL-10对脊髓损伤后NF-κB表达影响的研究.pdfVIP

Nervous Diseases and Mental Health，2008，Vo1．8，No．1 论著 IL一10对脊髓损伤后NF—KB表达影响的研究 雷德强 赵洪洋 张方成 刘如恩 邓兴力 【摘要】 目的 研究II 一1O对脊髓损伤后核因子NF—KB表达的影响。方法 5O只雌性 SD大 鼠随机分成实验组和对照组，在手术显微镜下完成脊髓半切损伤模型；每一小组在伤后半小时分别给予 腹腔注射，在不同时间点快速取出伤段脊髓液氮冻存；常规方法提取脊髓组织核蛋白完成核蛋白定量； 通过Trans AMTM NF—KB P65试剂盒检测NF—KB P65的表达。结果 实验组和对照组各组脊髓组 织中均存在NF—KB P65表达且表达差异具有统计学意义 (P0．01)。实验纽各小组NF—KB P65的 活性较对照组均不同程度受到抑制；IL一1O对各时间点(1，4，6，12，24 h)半切损伤后脊髓组织中NF— KB p65表达的抑制率分别是12．0 ，28．4 ，23．8 ，15．2 ，17．4 。结论 IL一1O对脊髓损伤后炎 症介质核因子NF—KB表达有抑制作用。 【关键词】 白介素一10； 脊髓损伤； 早期炎症； 核因子一KB Effects of interleukin--10 for the expression of NF—KB in spinal cord injury LEI De—qiang，ZHA0 Hong—yang，ZHANG Fang—cheng，et a1． Department of Neurosurgery，Union Hospital，Tong ji Medical College，Hua zhong University of Science and Technology，Wuhan 430022，China [Abstract】 0bjective To investigate the implications and effects of IL一10 for the expression of NF—KB in injured spinal cord．Methods The rats(SD，female)randomly divide into experimental group and controlled group．A model of spinal cord inj ury was completed with an operation microscope．Half hour later，each group had been administered peritoneally before the in~ured spinal cord was instantly preserved in liquid nitrogen．The nucleoprotein of myeloid tissue was extracted in routine way and quan— titated the nucleoprotein．The expression level of NF—KB P65 in myeloid tissue was analyzed with a kit (Trans AMTM NF—KB P65) Results The expression of NF—KB P65 exists in myeloid tissue of the experimental group and the control group，the expression difference was significant(P 0．O1)．The ac— tivity of NF——KB P6 5 in the administered experimental group was to some degree restricted compared with control group，the inhabitation ratio by IL一1O in injured myeloid tissue at different intervals(1，4， 6，12 and 24 h)are：12．0 ，28．4 ，23．8