微流体功能芯片检测装置在流感病毒检测中的初步研究-病毒病所.PDF

·8 · 公共卫生与预防医学 2005 年第 16 卷第 4 期 　　J of Pub Health and Prev Med 　2005 , Vol . 16 , No. 4 ·论著 · 微流体功能芯片检测装置在流感病毒 检测中的初步研究 乐加昌　徐红 　张德元 　崔元波 　孟涛 　吴岚军 【摘要】目的　建立一种新型有效 、快速 、灵敏的呼吸道病毒检测技术 。方法 　用微流体生物功能芯片微 量检测系统检测传染性强 、潜在世界大流行危害的流感病毒 。结果 　该技术检测限度约在病毒粒子 104 。结 论 　初步研究表明微流体生物功能芯片是一种快速 、灵敏的检测系统 ,对病原微生物感染 ,尤其是对经空气传 播的病毒的快速检测辅助临床诊断具有重要作用 。此外在控制病房的院内感染也将发挥其作用 。 【关键词】微流体功能芯片 ; 流感病毒 + ( ) 　　 　【中图分类号】R - 331 ;R373 . 1 　　【文献标识码】A 　　【文章编号 2005 The Study of Microliquid Biosensor Chip Device for Detecting Detect Influenza Viruses 　　YUE J iaChang , X U Hong , ZHAN G DeYuan , M EN G Tao , WUL anJ un. 　1. Institute of B iop hysics , Chinese Academy of Sciences , B eij ing 　100101, China 【Abstract 】　Objective 　To establish a new method technique to detect respiratory track viruses rapidly and sensitive ly . Methods 　The influenza viruse , which is strongly infectious and potentially pandemic infectivity quickly and cause , was detected by Microliquid Biosensor Chip System. Results 　The result showed that the limitation of detection was about 104 of viral particle . The samples of influenza virus were detected and show limit of detection about 104 of viral particles. Conclu sion 　The Microliquid Biosensor Chip System is a rapid and sensitive technique and will be useful for detecting pathogens of respiratory viruses and assisting assistant clinical virus diagnosis. In addition , it would also play a important role in the con trol of hospital infection . 【Key words】　Microliquid biosens