4个绵羊品种PTGS2基因部分片段的多态性分析.pdfVIP

安徽农业大学学报，2008，35(1)：21—25 Journal of Anhui Agricultural University 4个绵羊品种PTGS2基因部分片段的多态性分析 王训翠 ，储明星 ，陈宏权 ，朱国祺 ，方 丽 ，叶素成 (1．安徽中医学院科研处，合肥230038； 2．中国农业科学院北京畜牧兽医研究所，中国农业科学院家养动物遗传资源与种质创新重点开放实验室，北京100094； 3．安徽农业大学动物科技学院，合肥230036) 摘 要：设计2对引物，采用PCR—SSCP和PCR—RFLP技术检测前列腺素内过氧化物合酶2(PTGS2)基因在小 尾寒羊、湖羊、多赛特羊、萨福克羊4个绵羊品种304个个体中的单核苷酸多态性。结果表明，PTGS2基因引物1 扩增片段在4个绵羊品种中不存在PCR—SSCP多态性。引物2扩增片段存在PCR—RFLP多态性，在4个绵羊品种 中都检测到2种基因型(AA、AB)，都没有检测到BB基因型。A等位基因频率明显高于B等位基因频率。小尾寒羊与 多赛特羊、萨福克羊、湖羊之间PTGS2基因型分布差异显著(P0．05)，其余品种之间PTGS2基因型分布差异都不 显著(P0．05)。 关键词：绵羊；前列腺素内过氧化物合酶2基因；多态性 中图分类号：$826．2 文献标识码：A 文章编号：1672—352X(2008 J01-0021-05 Polymorphism analysis of partial fragment of PTGS2 gene in four sheep breeds WANG Xun—cui ，CHU Ming—xing ，CHEN Hong quan。ZHU Guo—qi ，FANG Li ，YE Su．cheng。 ， (1．Scientific Research Department，Anhui College of Traditional Chinese Medicine，Hefei 230038； 2．The Key Laboratory of Domestic Animal Genetic Resources and Germplasm Innovation of CAAS，Institute of Animal Science， Chinese Academy of Agricultural Sciences，Beijing 100094； 3．Sqhool of Animal Science and Technology，Anhui Agricultural University，Hefei 230036) Abstract：The single nucleotide polymorphism of prostaglandin—endoperoxide synthase 2(PTGS2)gene was detected in four sheep breeds(Small Tail Han，Hu，Dorset and Suffolk)by PCR-SSCP and PCR-RFLP with two pairs of primers．The results indicated that there was no PCR—SSCP polymorphism in four sheep breeds detected by primer 1．There was PCR-RFLP polymorphism in four sheep breeds detected by primer 2．Two genotypes(AA and AB)were detected in four sheep breeds and BB genotype was not detected．Frequency of allele A was obviously high— er than that of allele B．The PTGS2 genotype distributions were significantly different(P0．05)among Small Tail Han