利用高灵敏的TSA_FISH在玉米中定位bz1_bz2基因_英文_.pdf

Develop ment al Repro ductive Biolo gy IS SN 　CN 113569Q ( ) 11 1 :1～7 ,2002 　　　　　　　　　　　　　　　　　　　　　　　　　　 　　　 Chromosomal Localization of Genes bz 1 , bz2 in Maize by Using Ultrasensitive FISH with ( ) Tyramide Signal Amplif ication TSAFISH ① LI Zongyun ,NING Shunbin ,HAN Yonghua ,LIU Lihua ,SONG Yunchun ( Key L aboratory of MOEf or Plant Developmental B iology , Wuhan University , Wuhan 430072 , China) Abstract :It has been reported that endosperm undergoes programmed cell death ( PCD) during maize ( ) kernel development . Both bz 1 bronze and bz2 are anthocyanin biosynthetic genes ,and related to de velopment of aleuronic layer of maize seeds. Tyramide signal amplification fluorescence in situ hybridiza tion ( TSAFISH) is a novel and high sensitive FISH technique ,which is suitable for routine application in plant cytogenetic research . Using this technique ,we physically mapped the bz 1 gene onto the short arm of chromosome 9 and the long arm of chromosome 1 ;the percentage distances from centromere to hy bridization site were 40 . 2 ,75. 4 respectively ,and the bz2 onto the long arm of chromosome 1 and the short arm of chromosome 5 ;the percentage distances from centromere to hybridization site were 21. 6 ,