二乙烯三胺多齿配体的合成及DNA的切割活性2012.pdf

·380 · 药学学报 Acta Pharmaceutica Sinica 2012, 47 (3): 380−384 二乙烯三胺多齿配体的合成及DNA 的切割活性 1, 2 2 2* 朱消非 , 刘艳红 , 叶 勇 (1. 郑州大学学报编辑部, 河南 郑州 450001; 2. 郑州大学化学系, 河南省磷化工工程技术研究中心, 河南 郑州 450052) : , , 摘要 为了得到具有核酸切割功能的人工核酸酶 设计合成了二乙烯三胺单齿、双齿、四齿配体 目标化合 物的结构经红外、核磁氢谱、碳谱和电喷雾质谱确认。在磷酸氢钠-磷酸二氢钠缓冲溶液中对质粒 DNA 切割活 性的初步实验结果表明: 在多齿配体的锌 (II) 配合物存在下, 37 ℃保温 48 h 后, 质粒 DNA 由 Form I 断裂为 Form II, 即合成的目标化合物有明显的切割活性。通过 T4 连接酶实验证明了目标化合物对 DNA 的切割为水解 切割机制。 关键词: 二乙烯三胺; 多齿配体; 质粒 DNA; 切割试剂 中图分类号: R916 文献标识码: A 文章编号: 0513-4870 (2012) 03-0380-05 Synthesis of diethylenetriamine polydentate ligands and their DNA-cleaving activity 1, 2 2 2* ZHU Xiao-fei , LIU Yan-hong , YE Yong (1. Editorial Board of Journal of Zhengzhou University, Zhengzhou 450001, China; 2. Phosphorus Chemical Engineering Research Center of Henan Province, Department of Chemistry, Zhengzhou University, Zhengzhou 450052, China) Abstract : A series of multinuclear diethylenetriamine ligands were synthesized and used as artificial nuclease enzyme model. Target compounds were characterized by 1H NMR, 13C NMR, IR and ESI-MS. Preliminary studies on the cleavage of pUC19 DNA in the presence of metal complexes have also been performed and the results revealed that these complexes could act as powerful catalysts for the cleavage of pUC19 DNA after 48 h under physiological conditions. The hydrolytic cleavage mechanism of DNA plasmid by title compound was