中药郁金的指纹图谱的初步研究.doc

中药郁金的指纹图谱的初步研究 摘要：目的　建立温莪术挥发油与姜黄素的指纹图谱，对郁金与莪术的指纹特征进行比较,探索二者之间的内在化学本质区别，为有关的药学研究和生产的质量控制提供技术基础。方法　采用水蒸气蒸馏法提取挥发油，加热回流法提取姜黄素；利用中药指纹图谱技术建立指纹图谱，对郁金与温莪术的指纹图谱特征进行直观比较。结果　所建立了的指纹图谱，具有良好的精密度，重现性及稳定性。郁金与温莪术在指纹图谱特征上有明显的不同。结论　利用中药色谱指纹图谱技术控制郁金质量具有良好的可行性。 关键词：温莪术；郁金；挥发油；姜黄素；指纹图谱 Primary Studies of Radix Curcumae Fingerprints Yangshaui.shishuxia Abstract: Objective To establish Rhizoma curcumae wenyujin fingerprints of volatile oil and curcuminoids for the fingerprints characteristic comparison of Radix Curcumae kawangsiensis with Rhizoma curcumae wenyujin. Explores their differences of chemistry essence in order to effectively control The two drug quality, and provides the technology supports for quality control of their studies and production. Methods The volatile oil was extracted by the steam distillation; the curcuminoids was extracted by heating backflow law extraction. The fingerprints characteristics of Rhizoma curcumae wenyujin and volatile were compared after their fingerprints had been established. Results The established fingerprints were accuracy, reproducibility and the stability. The results shown there was obvious difference on the fingerprints characteristics of two Chinese traditional medicines. Conclusion It has the good practicability using the Chinese national medicine chromatograph fingerprints to control the curcuma quality. Key words Rhizoma curcumae wenyujin；Radix curcumae； Volatile oil；Curcumin；Fingerprints 前言 郁金为姜科姜黄属植物温郁金Curcuma.wenyujin Y.H.Chen et C.Ling. 姜黄C.longa L.广西莪术C.kwangsiensis S.G.Lee et C.F.Liang. 或蓬莪术C.phaeacaulis Val. 等品种的干燥块根[1]。本药性寒，味辛、苦，归心、肝、胆经，具有活血行气止痛、清心解郁、利胆退黄、凉血止血之功效，常用于经闭痛经、胸腹胀痛、黄胆尿赤等证。郁金药材不仅供中医临床配方使用，也是利胆排石片、郁金银屑片、舒肝和胃丸、安宫牛黄丸等多种中成药的原料。据统计中国药典2000年1-19册部颁标准中有 143个成药使用了郁金原料，约占国家标准收载中成药的3.3%[2]。国内对郁金从其资源分布、采收加工、化学成分,药理作用等方面多有研究[3～]，发现其含有挥发油、姜黄素类、多糖、生物碱，微量元素和其它化学成分。现代药理学证实郁金内有保肝、利胆、抗癌，抗自由基损伤和对中枢神经抑制等作用，并对血液系统和消化系统有影响[12～18][19～22]。中药指纹图谱在国内仍为中药研究的一大热点，继现有的中药注射液指纹图谱的完成，对中药的药材、饮片、其它制剂的指纹图谱研究正方兴未艾。 尽管郁金的研究较为广泛，但其目前仍有一药多名、数药同名等状况，其临床