dmso（DMSO）.docVIP

dmso（DMSO） Two methyl sulfoxide DMSO (cell culture level) American MPBIO Product description: DMSO A aprotic polar solvent used in chemical reactions, PCR reactions, and as a protective agent for cryopreservation of cells, tissues and organs. DMSO cells for freezing media can protect cells from mechanical damage caused by ice crystals. It can also be used for cryopreservation of embryonic stem cells (ESC) and hematopoietic stem cells, including primary cultures, sub cultures, recombinant aneuploid, hybridoma and other cell lines. Also, it is often used in combination with BSA or FBS. Use: (1) DMSO is widely used in low temperature protection of human and animal cell lines and bacteriophage lambda, and the steps of preparing DMSO solution to freeze cells are as follows: 1) Prepare frozen medium: contains culture medium for cells, 10-20% serum and 5-10% DMSO; 2) Digest adherent cells with trypsin or other suitable methods; (cells are best placed in logarithmic phase) 3) The cells were collected at low speed centrifugation (4 C, 250 x g, 10min), and the culture medium was removed; 4) The cell density was 106-107 cells/ml by suspending the cells in a frozen medium; 5) The average distribution of cells, into the frozen tube; 6) In accordance with standard cryopreservation procedures, frozen cells are frozen at temperatures of -70 degrees C or less; (2) when the cells were fused, 10% DMSO was added into the 40-50% PEG solution; (3) have reported that DMSO as a buffer component, is used in the following experiments: Poly (A+) RNA purification, E. coli competent cell transformation, PCR reaction, cDNA library, DEAE- amplification and DNA sequencing of dextran mediated transfection, Polybrene mediated DNA transfection; (4) corresponding procedures are described to recover DNA after membrane filtration using DMSO, for subsequent PCR amplification reactions; (5) capillary electrophoresis for DNA sequencing, mixing 5% DMSO (v/w) into 2 M urea, and adjusting DMSO concentration t