高效液相色谱 原子荧光法同时测定饮用水中甲基汞和乙基汞（Simultaneous determination of methylmercury and ethylmercury in drinking water by High Performance Liquid Chromatography Atomic Fluorescence Spectrometry）.docVIP

高效液相色谱 原子荧光法同时测定饮用水中甲基汞和乙基汞（Simultaneous determination of methylmercury and ethylmercury in drinking water by High Performance Liquid Chromatography Atomic Fluorescence Spectrometry） Simultaneous determination of methylmercury and ethyl mercury in drinking water by High Performance Liquid Chromatography Atomic Fluorescence Spectrometry Abstract: a method for determination of methylmercury and ethyl mercury in drinking water by solid phase extraction coupled with High Performance Liquid Chromatography Atomic Fluorescence Spectrometry (SPE-HPLC-AFS) was developed. The experimental method of solid phase extraction purification and concentration samples, using HPLC mobile phase acetonitrile containing 5% (v/v) -0.462% (v/v) -0.12% ammonium acetate (v/v) cysteine solution, the chromatographic column is C18 reversed-phase column. The detection limits for methylmercury and ethyl mercury were 0.56ng/L and 1.32ng/L, respectively. Keywords: drinking water, methylmercury, ethyl mercury Organomercury (such as methylmercury and ethylmercury etc.) of lipophilic toxins, mainly involving the central nervous system, can be absorbed through the respiratory tract, gastrointestinal and skin, with different inorganic mercury, the intestinal absorption rate is very high. No matter any way invasion can occur stomatitis; oral can cause acute gastroenteritis; neuropsychiatric symptoms of neurasthenia syndrome, mental disorders, delirium, paralysis, tremor, ataxia, concentric vision narrowing can occur; kidney damage can cause acute renal failure. In addition, it can cause heart, liver and skin lesions, [1]. At present, the determination of methylmercury and ethyl mercury in surface water and sewage by sulfhydryl cotton enrichment gas chromatography and HPLC-ICP- MS [1-3]. But the former due to the impurity in sample (mercaptan, thioether and thiophene, etc.) will influence the existence of column separation efficiency, determination of methylmercury interference; while the latter is simple,