激光共聚焦显微镜的原理与应用范围（Principle and application range of laser scanning confocal microscopy）.docVIP

激光共聚焦显微镜的原理与应用范围（Principle and application range of laser scanning confocal microscopy） Principle and application range of laser scanning confocal microscopy Laser scanning confocal microscope using laser as a light source, using confocal principle and device based on the traditional optical microscope and digital image processing, a set of observation, analysis and output system of the object observed by computer. The optical imaging resolution of 30%~40%, the use of ultraviolet or visible light excited fluorescence probe to obtain fluorescence image of cells or tissue microstructures, observe the changes of cell morphology, physiological signals and at the subcellular level on morphology, molecular biology, neuroscience, pharmacology, a new generation of genetic tool etc. in. Principle of 1 laser scanning confocal microscopy (LSCM) In principle, confocal microscopy is a modern optical microscope, which makes several improvements in the technical aspects of ordinary light mirrors: 1.1, using laser as light source, because the laser is very good monochromatic, light source beam of the same wavelength, fundamentally eliminate the color difference. 1.2 using a confocal technique, a baffle with small holes is placed on the focal plane of the objective lens to prevent stray light from the focal plane. The aberration is eliminated, and the chromatic aberration is further eliminated Using 1.3 point scanning technology will sample decomposed into numerous point two-dimensional or three-dimensional space, using a laser beam is very small (point source) by progressive scan imaging, and then through the computer into a whole plane or three-dimensional image. The traditional light microscope is imaged in the presence of the field light source. The image of each point in the specimen is disturbed by the diffraction light and scattered light of the adjacent point. The sharpness and precision of the two images are incomparable. 1.4 collect and process optical signals with a compu