质粒与载体（Plasmid vector）.docVIP

质粒与载体（Plasmid vector） Hits: 442 published: 2008-07-08 17:32 reprinted from Lilac Garden Source: Internet A plasmid Most organisms are in the form of DNA to store its genetic information. The genetic material to be passed to the first condition of life and growth in nature is its offspring must have at least one copy of the original (ORI origin, of replication, or the starting point, so that the whole genome duplication) to copy. Contains the copy of the original genetic material called replicon, let us translate it for replication! The original copy of the nuclear body is divided into a prokaryotic chromosome and plasmid (plasmids) and phage genome (phage genome) three. The similar plasmid genome and prokaryotic chromosomes, is composed of double refining DNA, and existed in the form of super crimp. Their genome from about 2000 to 150000 nucleotides, most in a ring, but there are very few is a linear structure (such as Borrelia burgdorfferi). In fact, you can see them as a relatively small prokaryotic chromosome. In the natural environment, they are quite common in prokaryotic cells, and related and many special features such as its host: Red bacillus (Shigella), the drug resistance of Rhizobium (Rhizobium) nitrogen fixation, Agrobacterium tumefaciens (Agrobacterium) induced tumor and Pseudomonas bacteria (Pseudomonas) on cyclic organic compounds the decomposition and so on. The following we talk to bacterial plasmids, especially gram negative bacteria plasmid. Type two, plasmid When we talk about the type of plasmid, to see which way you look at them, for example, gamogony resistance ability, host range and DNA replication etc.. There is no lateral association between these classification criteria. You cannot say with reproductive medicine or not plasmid anti drug resistance, but also unable to determine the host range and control plasmid copy of the relevance. We use these terms, just to do some specific plasmid traits described. Is not the real system classificat