武当松针茶中莽草酸的提取工艺优选及含量测定-安徽医药.DOCVIP

（1）为什么做2次正交试验？ 答复：首先感谢评审专家指出问题。本实验在前期设计时确有考虑不周之处，加之实验工作是由不同人员分别完成，从而得出了以甲醇和水作为提取溶剂时的最佳提取条件，文章在撰写时遵照原始数据，将2次正交试验结果分开来写，进而比较了两种不同提取溶剂的实验结果。 （2）饱和正交的方差分析，你是怎么做的。为什么不选一个参考的误差项。答复：饱和正交，因素水平，做9组实验，每组做3次平行实验，求平均值，得出实验结果。 “武当松针茶”中莽草酸的提取工艺优选及含量测定 摘 要 目的：优选“武当松针茶”中莽草酸的提取工艺，并建立其含量测定方法。方法：采用反相高效液相色谱法测定“武当松针茶”中莽草酸的含量。通过正交设计试验，分别以水和不同浓度的甲醇为提取溶剂考察料液比、超声功率和超声时间对莽草酸含量的影响。结果：莽草酸在10.0～10μg /ml（r=0.999）范围内呈良好的线性关系；平均加样回收率为99.%，RSD=%（n=6）。为超声功率200W提取45min为料液比1:，甲醇浓度40%，超声功率500W，超声时间45mi草酸的平均含量为mg/g、38.66mg/g。结论：，料液比1:0，建立的含量测定方法稳定可靠，可用于提取和测定“武当松针茶”中莽草酸的含量。 关键词：武当松针茶; 莽草酸；提取工艺；含量测定；高效液相色谱法 Extraction Technology Optimization and Content Determination of Shikimic Acid in ‘Wudang pineneedle tea’ ( 1. Department of Pharmacy, Dongfeng Hospital Affiliated to Hubei University of Medicine, Hubei Key Laboratory of Wudang Local Chinese Medicine Research, Hubei shiyan,442000；2.Hubei Wudang Biopharmaceutical Science and Technology Company Limited, Hubei shiyan,442000) ABSTRACT OBJECTIVE: To optimize the extraction technology of shikimic acid in ‘Wudang pineneedle tea’ and to establish the assay method. METHODS: The content of shikimic acid in ‘Wudang pineneedle tea’ was determined by RP-HPLC-DAD. Orthogonal design was used to investigate the influence of solid/liquid ratio, ultrasound power, ultrasound time using water and different concentration methanol as extraction solvent, respectively. RESULTS: A good linearity of shikimic acid was within the range of 10.02～101.μg/ml（r=0.999, and the average recovery was99.5%（RSD=%, n=6）.Extracted with water, the better extraction conditions by orthogonal experiment were ratio of solid to liquid 1:50, ultrasonic power 200 w, extraction of 45 min. Extracted by methanol, the better extraction conditions by orthogonal experiment were ratio of solid to liquid 1:50, 40% methanol concentration, ultrasonic power 500 w, ultrasonic time of 45 min.The average content of shikimic acid were 36.38 mg/g and 38.66 mg/g respectively. CONCLUSION: The optimal technology wa