彩虹明樱蛤多糖对转染hbv的hepg2细胞表达功能的影响.pdfVIP

Med J Chin PLA, Vol. 37, No. 2, February 1, 2012 　109 彩虹明樱蛤多糖对转染HBV 的Hep G2细胞表达功能的影响 向维，陈松华，吴媛媛，李仁富 [摘要]　 目的　研究彩虹明樱蛤多糖(M I P ) 对转染H B V 的H e p G 2 细胞表达功能的影响。方 法　以不同浓度 －2 4 (10 ~ 10 μg /ml )M IP作用于体外培养的Hep G2.2. 15细胞，采用MTT法、时间分辨免疫分析法(TRFIA )和ELISA 法检测 MIP对Hep G2.2. 15细胞的毒性作用及HBsA g 、HB eA g 、Pre- S 1A g表达的影响，采用FQ P CR检测MIP对HBV DNA 复制的 －2 2 －2 抑制作用。结果　M IP在10 ~ 10 μg /ml 浓度范围内对Hep G2 .2 . 15细胞无毒性作用，TC50 为4 633 μg /ml 。M IP在10 ~ 2 10 μg /ml 浓度范围内，对HBsA g 、HB eA g 、Pre- S 1A g 以及HBV DNA 均有一定抑制作用，相应的治疗指数分别为129 、 28 、20和83 。结论　MIP具有一定的抗HBV 活性。 [关键词]　彩虹明樱蛤多糖；肝炎, 乙型, 慢性；Hep G2细胞 [中图分类号]　R5 12.62 [文献标志码]　A [文章编号]　0577-7402 (20 12)02-0 109-04 Study of the effects of Moerella iridescens polysaccharide on hepatitis B virus transfected HepG2 cells * XIANG Wei, CHEN Song-hua , WU Yuan-yuan, LI Ren-fu Sanatorium of Nanjing General Hospital of Nanjing Command, Medical Rehabilitation Center of PLA , Nanjing 2 1113 1, China *Corresp onding auth or, E -mail : squirrel_ 123@ 126.com This w ork w as supp orted by th e National Natural Science Foundation of China [Abstract]　Objective　To inv estigate th e effects of Moerella iridescens p oly saccharide (MIP) on Hepatitis B v irus (HBV ) transfected Hep G2 cell s. Methods　Hep G2.2. 15 cell s w ere treated w ith different concentrations of MIP (10 －2 – 104 μg/ml), and th ey w ere cultured in vitro. Th e MTT assay, time-resolv ed fluoreimmunoassay (TRFIA ), and enzy me-linked immunosorb ent assay (ELISA ) w ere used to ex pl ore th e cy totox ic effect of MIP on Hep G2.2. 15 cell s and its effects on HBsA g, HBeA g, and Pre- S 1A g secrete